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将马铃薯Ｙ病毒中国分离株（ＰＶＹ－Ｃ）的６ｋＤ和ＮＩａ基因拼接和改造，分别构建了其正向表述和反向转录的植物表达载体。借助土壤农杆菌ＬＢＡ４４０４将在此双元载体上的６ｋＤ和ＮＩａ基因及新霉素磷酸转移酶基因（ＮＰＴⅡ）转入到烟草品种ＮＣ８９的染色体上，从而获得了抗卡那霉素的转化再生烟草植株。用ＰＶＹ－Ｃ对这些转化烟草进行抗性接种试验，结果表明部分转基因植株能够抵制ＰＶＹ－Ｃ的侵染，对抗病植株的子一代（Ｒ１）进行进一步的抗性分析发现，其Ｒ１代通过遗传也获得了这种抗病性。
The 6kD and NIa genes of potato Y virus Chinese isolate (PVY-C) were spliced and transformed, and their positive expression and reverse transcriptional plant expression vectors were constructed respectively. The 6kD and NIa genes and the neomycin phosphotransferase gene (NPTII) on this binary vector were transferred into the chromosome of tobacco variety NC89 by means of Agrobacterium tumefaciens LBA4404 to obtain transformed and regenerated tobacco against kanamycin Plant. The transgenic tobacco plants were tested for their resistance to PVY-C by inoculation with PVY-C. The results showed that some of the transgenic plants were able to resist the infection of PVY-C. Further resistance analysis of the offspring of the resistant plants (R1) Heredity also acquired this resistance.