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目的:观察朊蛋白(PrPc)在正常结肠粘膜、结肠炎及结肠癌组织中的表达,并探讨其临床意义。方法:采用免疫组织化学法分别检测PrPc在正常结肠组织、结肠炎及结肠癌组织(各40例)中的表达,并分析其在结肠癌及结肠炎组织中的表达与患者性别、分化程度、TNM分期、淋巴结转移及炎症程度等临床特征间的关系。结果:PrPc在正常结肠及结肠癌组织中均有表达,在结肠炎中表达较低,在结肠癌组织中阳性表达率为60%(24/40),明显高于正常结肠组织的35%(14/40)及结肠炎组织的30%(12/40)(P<0.05)。轻度结肠炎中PrPc的阳性表达率明显高于重度结肠炎,结肠癌中PrPc的表达与患者的肿瘤分级、分期显著相关(P<0.05)。结论:PrPc在结肠癌中的表达增高,在结肠炎中表达较低,可作为临床判断结肠癌恶性程度及结肠炎炎症程度的重要参考指标。
Objective: To observe the expression of prion protein (PrPc) in normal colonic mucosa, colitis and colon cancer and to investigate its clinical significance. Methods: The expressions of PrPc in normal colorectal tissue, colitis and colon cancer tissues (40 cases each) were detected by immunohistochemistry. The expression of PrPc in colorectal cancer and colitis tissues was analyzed with the gender, differentiation, TNM staging, lymph node metastasis and the degree of inflammation and other clinical features. Results: The expression of PrPc in normal colon and colon cancer tissues was lower than that in normal colorectal tissues, and it was lower in colitis (60%, 24/40) than that in normal colon tissues 14/40) and colitis tissue (12/40) (P <0.05). The positive expression rate of PrPc in mild colitis was significantly higher than that in severe colitis. The expression of PrPc in colorectal cancer was significantly correlated with the tumor grade and stage (P <0.05). Conclusion: The expression of PrPc in colorectal cancer is high, and it is low in colitis. It can be used as an important reference index for judging the malignant degree of colorectal cancer and colitis inflammation.