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以鸭梨、黄金梨、阿巴特梨和杜梨为试材,对梨组培过程中影响外植体褐化的因素及防褐措施进行了研究。结果表明,品种、采样时期以及外植体内酚类物质含量等因素都显著地影响材料褐化率。抗褐剂试验表明,培养基中添加0.2g/L的聚乙烯吡咯烷酮(PVP)或100mg/L抗坏血酸(Vc),或将外植体在200mg/L抗坏血酸水溶液中浸泡30min后接入添加2g/L活性炭的培养基,均能显著抑制鸭梨褐化的发生。低温试验表明,鸭梨外植体经4℃低温处理6h后接种,或接入初期在4℃低温中培养12～24h,褐化程度明显减轻。
Taking pear, yellow pear, abbott pear and pear as test materials, the factors influencing the browning of explants in pear tissue culture and the measures of preventing browning were studied. The results showed that factors such as variety, sampling period and content of phenols in explants significantly influenced the material browning rate. Anti-browning test showed that 0.2g / L polyvinylpyrrolidone (PVP) or 100mg / L ascorbic acid (Vc) was added to the medium or the explants were soaked in 200mg / L ascorbic acid solution for 30min and then added with 2g / L activated carbon medium, can significantly inhibit the occurrence of brown pear. Low-temperature experiments showed that the pear explants were inoculated with low temperature treatment at 4 ℃ for 6h, or cultured in low temperature at 4 ℃ for 12 ~ 24h, the browning degree was significantly reduced.