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对探讨ｐ１５基因甲基化在儿童急性白血病(ＡＬ)微小残留病灶(ＭＲＤ)诊断中的价值,采用甲基化特异性聚合酶链反应(ＭＳＰ)研究４９例初治儿童ＡＬ(包括ＡＬＬ３１例及ＡＭＬ１８例)和２０例对照组的ｐ１５基因甲基化情况。动态观察４例ＡＬＬ及２例ＡＭＬ治疗前后ｐ１５基因甲基化的变化。结果显示,儿童ＡＬ患者ｐ１５基因甲基化阳性率为６７．３%,ＡＬＬ与ＡＭＬ的阳性率(分别为６１．３%和７７．７%),差异无显著性:对照组２０例均阴性。动态观察的６例儿童ＡＬ,初诊时均呈现ｐ１５基因甲基化;完全缓解时,４例仍呈现甲基化,其中３例分别于３个月、５个月、６个月后复发:另一例甲基化转为阴性,未复发:ＭＳＰ敏感度可达１０－３。因此,ＭＳＰ检测ｐ１５基因甲基化有助于检测儿童ＡＬ的ＭＲＤ。
To investigate the value of methylation of p15 gene in the diagnosis of minimal residual disease (MRD) in children with acute leukemia (AL), methylation-specific polymerase chain reaction (MSP) AML18 cases) and 20 cases of control group p15 gene methylation. Dynamic changes of methylation of p15 gene in 4 ALL patients and 2 AML patients were observed. The results showed that the positive rate of methylation of p15 gene in children with AL was 67.3%, the positive rate of ALL and AML was 61.3% and 77.7%, respectively, with no significant difference: 20 cases in the control group were negative . Dynamic observation of 6 cases of children with AL, p15 gene methylation was present at the first visit; when complete remission, 4 cases still showed methylation, of which 3 cases were at 3 months, 5 months, 6 months after the recurrence: the other A case of methylation turned negative, no recurrence: MSP sensitivity of up to 10-3. Therefore, MSP detection of p15 gene methylation helps detect MRD in children with AL.