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目的 研究人胰腺癌细胞中ｎｍ２３ ｍＲＮＡ的表达与其转移潜能的关系。 方法 在建立不同转移潜能的人胰腺癌ＪＦ３０５ 单克隆细胞亚系的基础上，应用定量逆转录多聚酶链式反应技术（ＲＴＰＣＲ）检测不同转移潜能的人胰腺癌ＪＦ３０５ 单克隆细胞亚系细胞中ｎｍ２３ ｍＲＮＡ 的表达。 结果 ｎｍ２３Ｈ１ 基因ｍＲＮＡ在低转移潜能的ＪＦ３０５Ｃ细胞系中的表达（１－９０ ±０－０９）明显高于其在高转移潜能的ＪＦ３０５Ａ 细胞系中的表达（１－６３±０－１０，Ｐ＜ ０－０１）；ｎｍ２３Ｈ２ 基因ｍＲＮＡ的表达，在高、低转移潜能的细胞亚系间差异无显著意义（０－７９ ±０－１０ ，０－７７±０－０６，Ｐ＞０－０５）。 结论 ｎｍ２３ 基因，主要是ｎｍ２３Ｈ１ 基因，在人胰腺癌中起转移抑制作用
Objective To study the relationship between the expression of nm23 mRNA and its metastatic potential in human pancreatic cancer cells. METHODS: Based on the establishment of human pancreatic cancer JF305 monoclonal cell sublines with different metastatic potential, Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect human pancreatic cancer JF305 monoclonal cell sublines with different metastatic potentials. Nm23 mRNA expression. Results The expression of nm23-H1 mRNA in the JF305C cell line with low metastatic potential (1-90 ± 0-09) was significantly higher than that in the high metastatic potential JF305A cell line (1-63±0-10, P<0-01); nm23H2 mRNA expression was not significantly different between high and low metastatic potential subpopulations (0-79±0-10, 0-77±0-06, P>0 -05). Conclusion The nm23 gene, mainly the nm23H1 gene, plays a role in metastasis inhibition in human pancreatic cancer.