目的:建立同时测定祛风止痛片中原儿茶酸和原儿茶醛含量的方法。方法:采用RP-HPLC法,色谱条件为LunaC18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-0.2%磷酸溶液(16:84),流速:0.8mL·min-1,柱温:30℃,检测波长:280nm。结果:原儿茶酸和原儿茶醛分别在0.62～12.40μg·mL-1(r=0.9997)和0.14～2.80μg·mL-1(r=0.9997)浓度范围内均有良好的线性关系,平均加样回收率分别为96.9%、97.9%;RSD均小于2.0%。结论:本方法准确,重复性好,可用于该制剂的质量控制。 Objective: To establish a method for simultaneous determination of protocatechuic acid and protocatechuic aldehyde in Qufengzhitong Tablet. Methods: The chromatographic conditions were as follows: Luna C18 column (4.6 mm × 250 mm, 5 μm) with acetonitrile-0.2% phosphoric acid solution (16:84) at a flow rate of 0.8 mL · min- 30 ℃, detection wavelength: 280nm. RESULTS: Both protocatechuic acid and protocatechuic aldehyde showed good linearity in the range of 0.62 ~ 12.40μg · mL-1 (r = 0.9997) and 0.14 ~ 2.80μg · mL-1 (r = 0.9997) The average recoveries were 96.9% and 97.9%, respectively; RSDs were less than 2.0%. Conclusion: This method is accurate and reproducible and can be used for the quality control of this preparation.