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目的:了解ICAM-1和CD44v6在结、直肠癌中的表达,探讨它们与结直肠癌侵袭与转移的关系。方法:应用免疫组织化学pv-9000二步法检测ICAM-1和CD44v6在40例结直肠癌和30例大肠良性腺瘤组织中的表达情况,并分析其与结直肠癌临床病理特征的关系。结果:40例结、直肠癌和30例大肠良性腺瘤中,ICAM-1的表达阳性率分别是72.5%(29/40)、33.3%(10/30),CD44v6的表达阳性率分别是88.9%(27/40)、26.7%(8/30)。ICAM-1和CD44v6的表达与年龄、性别、肿瘤细胞分化程度无关,但与淋巴结转移、Dukes分期具有相关性(p<0.05)。有淋巴结转移、Dukes分期在C+D期者,ICAM-1和CD44v6的表达阳性率均明显升高。ICAM-1和CD44v6在大肠良性腺瘤中呈低表达或不表达。ICAM-1和CD44v6两者均阳性表达时,其预测结、直肠癌淋巴结转移的特异性升高,有一定的临床意义。结论:CD44v6和ICAM-1的阳性表达预示结直肠癌具有较强的侵袭转移能力,可作为预测结直肠癌转移潜能的生物学指标。
OBJECTIVE: To investigate the expression of ICAM-1 and CD44v6 in colorectal cancer and their relationship with invasion and metastasis of colorectal cancer. Methods: The expression of ICAM-1 and CD44v6 in 40 colorectal carcinomas and 30 colorectal benign adenomas was detected by immunohistochemistry pv-9000 two-step method. The relationship between ICAM-1 and CD44v6 in clinicopathological features of colorectal cancer was analyzed. Results: The positive rates of ICAM-1 in 40 cases of colorectal cancer and 30 cases of colorectal adenoma were 72.5% (29/40) and 33.3% (10/30), respectively. The positive rates of CD44v6 expression were 88.9 % (27/40), 26.7% (8/30). The expression of ICAM-1 and CD44v6 was not related to age, sex and tumor cell differentiation, but was correlated with lymph node metastasis and Dukes staging (p <0.05). With lymph node metastasis, the positive expression rates of ICAM-1 and CD44v6 in Dukes stage were significantly higher in C + D stage. ICAM-1 and CD44v6 were low or not expressed in benign adenoma of large intestine. When both ICAM-1 and CD44v6 are positively expressed, their specificities for predicting the lymph node metastasis of node and rectal cancer are of certain clinical significance. Conclusion: The positive expression of CD44v6 and ICAM-1 indicates that colorectal cancer has a strong invasion and metastasis, which can be used as a biological index to predict the metastatic potential of colorectal cancer.