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目的:通过检测转染细胞的活性,Meta分析超顺磁性氧化铁纳米(superparamagnetic iron oxide,SPIO)在分子影像学实验中用于转染细胞的最佳浓度区间。方法:系统检索国外(Pub Med、Embase、Cochrane Library)及国内主要数据库(万方、维普、中国知网及中国生物医学数据库)建库至2016年3月间关于SPIO转染细胞的最佳浓度区间的相关文献,结合Cochrane协作网的推荐,制定纳入研究文献的标准,对纳入文献进行质量评估并提取研究数据。采用STATA 12.0进行统计学分析:偏倚性检验(Begg’s检验)、异质性检验(I2、P值、Q值检验法),在异质性较大时行亚组分析,讨论各组异质性(I2、P值、Q值检验法),计算各组死细胞比率均差值在各浓度上的合并效应量及95%可信区间,并画出SPIO浓度对细胞活性影响的剂量反应曲线,获取最佳浓度区间。结果:共14篇国内外研究论著符合纳入标准,文献异质性检验I2=60.61%;P=2.675×10-9;Q=139.62,提示数据组间存在显著的异质性,故进一步行亚组分析,按SPIO修饰或标记材料不同分为4组[A组:SPIO-聚乙烯酰胺(PEI)组;B组:SPIO-多聚赖氨酸(PLL)组;C组:SPIO-葡聚糖组;D组:SPIO-探针组],各组数据无明显异质性;拟合各组各浓度节点死细胞比率均差值及95%可信区间,画出剂量反应曲线(三次样条插值拟合曲线),分别得到A至D组较适宜的浓度区间值:4~6、10~50、10~50、5~25μg/m L。Deeks漏斗图呈较对称漏斗形,P=0.23>0.1,表明数据无明显发表偏倚。结论:在分子影像学研究中,当SPIO由聚乙烯酰胺(PEI)、葡聚糖、葡聚糖-多聚赖氨酸(PLL)包裹或制备为探针时,以SPIO对细胞的毒性影响为主要衡量指标,标记细胞的适宜浓度区间分别为:4~6、10~50、10~50、5~25μg/m L。
OBJECTIVE: To detect the activity of transfected cells and analyze the optimal concentration range of superparamagnetic iron oxide (SPIO) for transfecting cells in molecular imaging experiments. METHODS: The optimal concentration of SPIO-transfected cells was systematically searched from the database of Pub Med (Embase, Cochrane Library) and major domestic databases (Wanfang, VIP, CNKI and China Biomedical Database) to March 2016 The relevant literature in the interval, combined with the recommendations of the Cochrane Collaboration, establishes criteria for inclusion in the research literature, performs quality assessments of the incorporated literature, and extracts research data. Statistical analysis was performed with STATA 12.0: Begg’s test, heterogeneity test (I2, P value and Q test), and when heterogeneity was large, subgroup analysis was conducted to discuss the heterogeneity of each group (I2, P value, Q value test method), calculate the combined effect of the difference in the ratio of dead cells in each group and the 95% confidence interval, and dose-response curve of the effect of SPIO concentration on cell activity, Get the best concentration range. Results: A total of 14 research articles at home and abroad met the inclusion criteria, the literature heterogeneity test I2 = 60.61%; P = 2.675 × 10-9; Q = 139.62, suggesting that there is significant heterogeneity among the data sets, Group analysis was divided into 4 groups according to the SPIO modified or labeled materials [group A: SPIO-polyethylamide (PEI) group; group B: SPIO-polylysine (PLL) group; D group: SPIO-probe group]. There was no significant heterogeneity in the data of each group. The difference between the dead cells in each group and the 95% confidence interval were fitted, and the dose response curve The interpolation curve fitting curve), respectively, the suitable concentration interval values of A to D group were obtained: 4-6, 10-50, 10-50, and 25-25 μg / m L respectively. The Deeks funnel showed a more symmetrical funnel shape with P = 0.23> 0.1, indicating no significant publication bias. CONCLUSIONS: In molecular imaging studies, when SPIO is encapsulated or prepared as a probe by polyethylamide (PEI), dextran, dextran-polylysine (PLL), the cytotoxic effect of SPIO on cells As the main measurement index, the suitable concentration range of labeled cells were 4 ~ 6,10 ~ 50,10 ~ 50,5 ~ 25μg / m L, respectively.