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1、1956—1957年从福州市郊耕作地采集131个土样,分离得62个菌株,经过鉴定结果,属于Azotobacter Chroococcum48株,Azotobacter Berijerinckii12株,Vzoiotobacter vinelandii1株,另一株可能是新种,因其从“123”号土样中分离得,故暂称“123”固氮菌。 2、我们分离到的菌种,通过固氮能力的测定,一般用一克甘露醇能固定5—6毫克的氮素,个别菌种的固氨能力较高,利用一克甘露醇,能固定7.9毫克的氮素。 3、自生固氮菌和 Pseudomonas Radiobacter 混合培养可以提高其固氮作用,一般能增加固氮效率10%以上。 4、实验结果指出自生固氮菌与土壤反应的关系的一般规律,在一定土壤反应范围内,自生固氮菌在土壤中的分布,随着士壤的 PH 值而转移,总的趋势是土壤 PH 值愈低自生固氮菌的分布愈少。 5、实验结果指出,自生固氮菌在土壤中的分布,与耕地种类有关,自生固氮菌一般在菜园土中分布较广,其次为水旱作田土,果园土最少。
1. From 1956 to 1957, 131 soil samples were collected from 62 villages in Fuzhou suburbs. 62 strains were isolated. After identification, the strains belong to Azotobacter Chroococcum 48, Azotobacter Berijerinckii 12 and Vzoiotobacter vinelandii 1, and the other strain may be a new species because of From “123” soil samples isolated, it is tentatively called “123 ” Azotobacter. 2, we isolated strains, through the determination of nitrogen fixation, usually with 1 gram of mannitol can be fixed 5-6 mg of nitrogen, individual strains of high ammonia capacity, the use of one gram of mannitol, can be fixed 7.9 Mg of nitrogen. 3, Autotrophic Azotobacter and Pseudomonas Radiobacter mixed culture can increase their nitrogen fixation, nitrogen fixation can generally increase the efficiency of 10%. The results showed that the distribution of autotrophic Azotobacter in soil was within the scope of certain soil reaction and shifted with the pH of soil soil. The general trend was the soil pH The less autotrophic Azotobacter distribution. The experimental results indicated that the distribution of autotrophic Azotobacter in the soil was related to the cultivated land types. Autotrophic Azotobacter was generally distributed widely in vegetable garden soil, followed by water and soil as the soil, with the least amount of orchard soil.