论文部分内容阅读
以５×１０３ｃｆｕ／鼠的新型隐球菌给小鼠脑室感染（ｉｃ）１ｈ后，分别口服氟康唑（Ｆｌｕ）５和１０ｍｇ／（ｋｇ·ｄ）或酮康唑（Ｋｅｔ）５０和１００ｍｇ／（ｋｇ·ｄ），ｂｉｄ×９ｄ。对ｉｃ小鼠连续观察４０天的存活率和平均存活时间，Ｆｕｌ分别为５０％～７０％和８～１５天，Ｋｅｔ是３０％～４０％和５～９天。由小鼠侧尾静脉注射１０６ｃｆｕ／鼠新型隐球菌３天后产生的全身感染，分别给小鼠口服Ｆｌｕ２．５～１０ｍｇ／（ｋｇ·ｄ）或Ｋｅｔ２５～１００ｍｇ／（ｋｇ·ｄ），ｂｉｄ×９ｄ。感染２４、２８和３２天的ＰＤ５０Ｆｌｕ是２．７、３．５和６．７ｍｇ／ｋｇ，Ｋｅｔ为２８．４、７０．４和８３ｍｇ／ｋｇ；Ｆｌｕ和Ｋｅｔ的平均存活时间分别是２３～３４天和２０～３１天。在上述两种实验模型中分离脑和肺做细菌培养，Ｆｌｕ均与对照和５０ｍｇ／（ｋｇ·ｄ）的Ｋｅｔ比较，对ｉｃ１０天及静脉注射感染１３天的小鼠，口服５和１０ｍｇ／（ｋｇ·ｄ）或２．５～１０ｍｇ／（ｋｇ·ｄ）Ｆｌｕ均显著抑制新型隐球菌生长。
Rats were challenged with 5 × 10 3 cfu / mouse Cryptococcus neoformans (ic) for 1 h before oral administration of Fluconazole (Flu) 5 and 10 mg / (kg · d) or Ket 50 and 100 mg / kg · d), bid × 9d. The survival rate and mean survival time of 40-day continuous observation of ic mice were 50% -70% and 8-15 days for Ful, 30% -40% for Ket and 5-9 days for Ket. The mice were infused with Flu2.5 ~ 10mg / (kg · d) or Ket25 ~ 100mg / (kg · d) orally respectively for 10 days after mice were injected with 106cfu / Cryptococcus neoformans for 3 days. . PD50Flu at 24, 28, and 32 days were 2.7, 3.5, and 6.7 mg / kg, Ket was 28.4, 70.4, and 83 mg / kg; mean survival time for Flu and Ket was 23-34 Days and 20 ~ 31 days. Brain and lung were isolated from the two experimental models for bacterial culture. Flu was compared to control and Ket at 50 mg / (kg · d). On days 10 and 10, mice were challenged with 5 and 10 mg / kg · d) or 2.5 ~ 10 mg / (kg · d) Flu significantly inhibited Cryptococcus neoformans growth.