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背景:目前对Fas有关的死亡区蛋白(Fas-associateddeathdomainpro-tein,FADD)、Fas有关的磷酸酶(Fas-associatedphosphatase-1,FAP-1)研究较少,尤其它们在脑缺血再灌注中的表达情况尚不清楚。目的:探讨脑缺血再灌注后表达的时间及表达部位。设计:随机对实验动物分组。地点和材料:本研究的地点为济南军区总医院神经内二科,健康、雄性Wistar大鼠,体重320g左右。由山东省卫生防疫站实验动物中心提供。干预:用线栓法制作大鼠大脑中动脉缺血再灌注模型,用免疫组化法检测FADD,FAP-1的表达。主要观察指标:FADD,FAP-1在缺血再灌注后鼠脑中的表达。结果:脑缺血前无FADD,FAP-1的表达,缺血再灌注后即有阳性染色,缺血再灌注12h表达最多(FADD:皮层11.60±0.57,纹状体6.27±0.72,海马14.06±1.35。FAP-1:皮层6.81±1.36,纹状体4.36±0.58,海马12.35±1.21),缺血再灌注24h阳性表达最少。海马区与皮层FADD,FAP-1的表达明显多于纹状体区。结论:大鼠脑缺血再灌注引起了FADD,FAP-1的表达,FADD,FAP-1的表达可能与凋亡有关。
BACKGROUND: Currently, there are few studies on Fas-associated death domain protein (FADD) and Fas-associated phosphatase-1 (FAP-1), especially in cerebral ischemia-reperfusion The situation is not clear. Objective: To explore the time and expression site after cerebral ischemia-reperfusion. Design: Randomly group experimental animals. Location and Materials: The study was conducted in Jinan Military Region General Hospital neurological two subjects, healthy, male Wistar rats weighing about 320g. Provided by the Shandong Provincial Health and Epidemic Prevention Laboratory Animal Center. Intervention: Rat middle cerebral artery occlusion (MCAO) model was established by thread occlusion. The expression of FADD and FAP-1 was detected by immunohistochemistry. MAIN OUTCOME MEASURES: Expression of FADD and FAP-1 in rat brain after ischemia-reperfusion. Results: There was no expression of FADD and FAP-1 before cerebral ischemia. The expression of FAP-1 was positive after ischemia-reperfusion. The expression of FAPD was highest at 12h after ischemia-reperfusion (FADD: cortex 11.60 ± 0.57, 1.35.FAP-1: cortex 6.81 ± 1.36, striatum 4.36 ± 0.58, hippocampus 12.35 ± 1.21), 24h after ischemia-reperfusion the least. Hippocampus and cortex FADD, FAP-1 expression was significantly more than the striatum. CONCLUSION: FADD and FAP-1 expression are induced by cerebral ischemia-reperfusion in rats. The expression of FADD and FAP-1 may be related to apoptosis.