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目的:利用小鼠黑色素瘤细胞B16,研究静磁场对肿瘤细胞生长和氧化应激的影响,探讨氧化应激介导静磁场影响肿瘤细胞生长的机制,为磁场在肿瘤疾病的治疗中的应用提供理论依据。方法:采用MTT法测定磁场对B16细胞活力的影响;利用流式细胞仪测定静磁场暴露对B16细胞周期分布的影响;利用生物化学方法测定磁场暴露对细胞氧化防御系统相关蛋白酶活性的影响。结果:24 h内50 m T-200 m T静磁场暴露可以抑制B16生长,但超过24 h的磁场暴露可以促进B16生长;100 m T和200 m T静磁场暴露对B16的细胞周期分布没有影响;B16暴露于100 m T和200 m T静磁场48 h,GST活性和GSH/GSSG水平表现为先上升后下降,SOD活性和T-AOC水平先下降后上升,CAT活性没有受到影响。结论:50 m T-200 m T静磁场可以抑制小鼠黑色素瘤细胞B16的生长,诱导肿瘤细胞产生氧化应激。
OBJECTIVE: To study the effect of static magnetic field on tumor cell growth and oxidative stress by using B16 in mouse melanoma cells, and to explore the mechanism by which oxidative stress mediates the effect of static magnetic field on the growth of tumor cells and provide the application of magnetic field in the treatment of tumor diseases Theoretical basis. Methods: The effect of magnetic field on the viability of B16 cells was measured by MTT method. The effect of static magnetic field exposure on the cell cycle distribution of B16 cells was measured by flow cytometry. The effect of magnetic field exposure on the activity of protease related to cell oxidative defense system was measured by biochemical methods. Results: Magnetic field exposure of 50 m T-200 m T within 24 h could inhibit the growth of B16, but the magnetic field exposure of more than 24 h could promote the growth of B16. The static magnetic field exposure of 100 m T and 200 m T had no effect on the cell cycle distribution of B16 ; B16 exposed to 100 m T and 200 m T static magnetic field for 48 h, GST activity and GSH / GSSG levels showed first increased and then decreased, SOD activity and T-AOC levels first decreased and then increased, CAT activity was not affected. Conclusion: The static magnetic field of 50 mT-200 mT can inhibit the growth of B16 in mouse melanoma cells and induce the oxidative stress in tumor cells.