本研究在国内首次从带毒香菇的菌丝体中分离和纯化出一种30nm 的球状病毒颗粒,经核酸鉴定为双键 RNA 病毒。病毒衣壳蛋白分子量为22 000道尔顿。并建立了一套从香菇中提取,纯化病毒颗粒和核酸型鉴定的方法。该研究结果为进一步研究我国香菇病毒的分布类型、传播途径及建立病毒检测系统奠定了技术基础,填 This study for the first time in China from mycelium with poisonous mushroom isolated and purified a 30nm spherical virus particles, the nucleic acid identified as double-stranded RNA virus. The virus capsid protein has a molecular weight of 22,000 Daltons. And established a set of mushrooms from the extraction, purification of virus particles and nucleic acid type identification method. The results of this study laid a technical foundation for further research on the distribution types and transmission routes of the virus and the establishment of a virus detection system in our country.