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目的研究6种中药单体对耐甲氧西林金黄色葡萄球菌(MRSA)的抗菌作用。方法采用微量肉汤稀释法测定苯唑西林对MRSA的MIC,应用PBP2’胶乳凝集试剂盒对MRSA所产的PBP2’蛋白进行鉴定。采用微量肉汤稀释法测定6种中药单体对MRSA的最低抑菌浓度(MIC)。结果经PBP2’鉴定,16株临床分离菌株均为MRSA。大黄酸的抗MRSA作用最强,MIC在4～16μg/ml,其次为小檗碱,而黄芩苷,黄芩素和穿心莲内酯抗MRSA的作用较弱,绿原酸对MRSA没有抗菌作用。结论大黄酸对MRSA的抗菌作用最强,有望成为抗MRSA的候选药物。
Objective To study the antibacterial effect of 6 kinds of traditional Chinese medicines on methicillin-resistant Staphylococcus aureus (MRSA). Methods The MICs of oxacillin against MRSA were determined by the broth microdilution method. The PBP2 ’protein produced by MRSA was identified by PBP2’ latex agglutination kit. The minimal inhibitory concentrations (MICs) of 6 kinds of Chinese herbal medicines on MRSA were determined by using the broth microdilution method. Results PBP2 ’identified 16 clinical isolates were MRSA. Rhein had the strongest anti-MRSA effect with MIC of 4-16 μg / ml, followed by berberine, while baicalin, baicalein and andrographolide had weaker anti-MRSA activity. Chlorogenic acid had no antibacterial effect on MRSA. Conclusion Rhein has the strongest antibacterial effect on MRSA and is expected to become a candidate drug for MRSA.