随着花药离体培养技术的发展,已可看出几方面的因素,对由小孢子里诱导植株的影响。本文介绍蔗糖浓度、pH值以及温度对枳壳和两种柑桔花药培养的影响。 With the development of in vitro anther culture techniques, several factors have been identified that have an impact on plants induced by microspores. This article describes the sucrose concentration, pH value and temperature of Citrus aurantium and two citrus anther culture.