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目的:观察远志清脑颗粒对拟痴呆模型小鼠学习记忆能力的影响及相关机制。方法:用ICR小鼠,设空白对照组,模型对照组,天保宁40 mg·kg-1组,远志清脑颗粒低、中、高剂量组(375,750,1 500 mg·kg-1),采用ip D-半乳糖(D-gal)合并亚硝酸钠(NaNO2)造痴呆模型小鼠。给药后通过水迷宫行为学实验测定模型小鼠的学习记忆能力,病理染色观察皮层和海马神经元的变化,分光光度法测定其脑组织生化代谢——超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力、丙二醛(MDA)含量。结果:远志清脑颗粒750 mg·kg-1组小鼠的学习能力和记忆能力与模型组相比显著提高(P<0.01);大脑SOD(1.64±0.26)U·mg-1较模型组(0.88±0.25)U·mg-1明显升高(P<0.01);GSH-Px(0.297±0.051)U·mg-1较模型组(0.179±0.035)U·mg-1升高(P<0.01);脑组织MDA(2.71±0.80)μmol·g-1较模型组(3.72±0.95)μmol·g-1显著降低(P<0.01)。结论:远志清脑颗粒明显提高小鼠的学习能力和记忆能力,并且可明显升高大脑SOD,GSH-Px活性,降低脑组织MDA含量,这可能是其改善学习能力和记忆能力的基础。
Objective: To observe the effect of Yuzhi Qingnao granule on the learning and memory abilities of mice model of dementia and its related mechanism. Methods: ICR mice were randomly divided into four groups: control group, model control group, 40 mg · kg -1 of Tianbao Ning group, and 375, 750, 1500 mg · kg -1 of Yuzhi Qingnao granule in low, medium and high dose groups D-galactose (D-gal) and sodium nitrite (NaNO2) were used to make mice model of dementia. After administration, the learning and memory abilities of model mice were determined by water maze behavioral test, the changes of cortex and hippocampal neurons were observed by pathological staining, and the biochemical metabolism of superoxide dismutase (SOD) Glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA) content. Results: Compared with model group, the learning and memory abilities of Yuanzhi Qingnao granule 750 mg · kg-1 group were significantly increased (P <0.01); the SOD of the brain was significantly higher (1.64 ± 0.26) U · mg-1 (P <0.01); GSH-Px (0.297 ± 0.051) U · mg-1 was significantly higher than that of model group (0.179 ± 0.035) U · mg-1 ). Compared with the model group (3.72 ± 0.95) μmol · g-1, MDA (2.71 ± 0.80) μmol · g-1 in brain tissue significantly decreased (P <0.01). CONCLUSION: Polygala tenuifolia will significantly improve the learning ability and memory ability of mice and increase the activity of SOD and GSH-Px in brain and decrease the content of MDA in brain tissue, which may be the basis of improving learning ability and memory ability.