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目的:探讨来氟米特(leflunomide,LEF)对佐剂性关节炎大鼠腹腔巨噬细胞IL-1,IL-6和 TNF-α分泌的影响及其抗炎、抗类风湿的可能作用机制.方法:大鼠足跖皮下注射Freund完全佐剂诱导关节炎模型;LEF灌胃后分次获取腹腔巨噬细胞,其培养上清液中IL-1,IL-6和TNF-α活性采用ELISA法或生物法测定.结果:佐剂性关节炎大鼠腹腔巨噬细胞IL-1,IL-6和TNF-α分泌较正常对照组明显升高;LEF对由 LPS诱导产生的 IL-1和 TNF-α有明显的抑制作用,作用产生快;LEF(10,25 mg/kg)在应用21天后对IL-6的分泌也有明显抑制作用.结论:来氟米特具有抑制佐剂性关节炎大鼠腹腔巨噬细胞 IL-1,IL-6和 TNF-α分泌水平的作用.
Objective: To investigate the effect of leflunomide (LEF) on the secretion of IL-1, IL-6 and TNF-α by peritoneal macrophages in rats with adjuvant arthritis and the possible mechanism of anti-inflammatory and anti-rheumatoid .Methods: The model of arthritis was induced by subcutaneous injection of Freund’s adjuvant into the foot of rats. The peritoneal macrophages were obtained by intragastric administration of LEF, and the activity of IL-1, IL-6 and TNF-αin culture supernatants was detected by ELISA IL-1, IL-6 and TNF-α secretion of peritoneal macrophages in adjuvant arthritis rats were significantly higher than those in normal control group. LEF induced the secretion of IL-1 and TNF-α significantly inhibited the production of IL-6 secretion.LEF (10,25 mg / kg) also inhibited the secretion of IL-6 after 21 days of application.Conclusion: Leflunomide can inhibit the adjuvant arthritis The role of IL-1, IL-6 and TNF-α secretion in rat peritoneal macrophages.