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作者用自泰国西部边境地区疟疾患者新分离的39株恶性疟原虫(P.f.)测试了阿齐霉素和双氢青蒿素(DHA)的体外抗疟活性,并同时测试了对氯喹、甲氟喹、奎宁和青蒿素的敏感性以便作相关性分析。体外药敏试验采用WHO评价裂殖体成熟抑制作用的标准方法,阿齐霉素测试的浓度范围为0.1-100μmol/L血,培养液混合物(BMM)DHA浓度范围为0.3-300 nmol/L BMM,青蒿素浓度为3-3 000 nmol/L BMM。自每个病人静脉采血后以RPMI1640培养基稀释至5％浓度,计数培养前虫血密度。药敏板每孔加
The authors tested the anti-malarial activity of azithromycin and dihydroartemisinin (DHA) in vitro against 39 Plasmodium falciparum (Pf) strains newly isolated from malaria patients in the western border region of Thailand and tested the efficacy of chloroquine, Quinine, quinine and artemisinin for correlation analysis. In vitro susceptibility testing was based on a WHO standard method of evaluation of schizonts maturation with azithromycin concentrations ranging from 0.1 to 100 μmol / L, and DHA concentrations ranging from 0.3 to 300 nmol / L BMM in the broth mixture (BMM) , Artemisinin concentration of 3-3 000 nmol / L BMM. Blood samples were taken from venous blood of each patient and diluted to 5% concentration with RPMI1640 medium. Agility plate added per hole