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本实验选用出生当天的彼得逊肉食鸡雏,将实验组右眼睑缝合作为实验眼,左眼为对照眼。于小鸡出生当天检测其屈光状态,用A超测量其前房深度、晶体厚度和眼轴长度,计算玻璃体腔长度。分别于小鸡出生后15、30、45天对小鸡作上述检查后处死实验组、空白对照组各10只,作病理切片观测鼻、颞侧及后极部巩膜软骨层、纤维层厚度,做相应部位巩膜软骨细胞、双核细胞计数。取45天小鸡实验、对照眼后极部巩膜做透射电镜切片。结果发现:小鸡被形觉剥夺后,实验眼出现明显轴性近视,眼轴延长主要以玻璃体腔延长为主;后极部巩膜软骨层变厚,纤维层变薄,巩膜软骨基质呈嗜酸性红染,提示有退行性变化。
The experimental selection of Peterson meat chickens on the day of birth, the experimental group right eyelid suture as the experimental eye, the left eye as the control eye. The refractive status of the chickens was measured on the day of birth, and the depth of the anterior chamber, the thickness of the anterior lens and the length of the ocular axis were measured by A-ultrasound and the length of the vitreous cavity was calculated. The chickens were sacrificed 15, 30 and 45 days after birth, respectively. After the above examination, 10 rabbits in the experimental group and 10 in the blank control group were sacrificed to observe the nasal, temporal and posterior pole scleral cartilage layers, the thickness of the fibrous layer, Do the appropriate parts of the scleral chondrocytes, binuclear cells count. Take 45 days of chick experiment, the control of the posterior eye sclera done by transmission electron microscopy. The results showed that there was significant axial myopia in the experimental eye after the deprivation of shape, and the prolongation of the axial length mainly occurred in the vitreous cavity. The posterior pole scleral cartilage layer became thicker, the fibrous layer became thinner and the scleral cartilage matrix was eosinophilic Red dye, suggesting a degenerative change.