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目的：研究核转录因子STAT3的诱骗寡核苷酸(decoy ODNs)对人膀胱癌细胞系增殖的抑制作用,并探讨其作用机制。方法：采用阳离子聚合物Sofast为转染试剂将STAT3 decoy ODNs转染入膀胱癌T24及5637细胞；用MTT法检测细胞增殖情况；流式细胞术检测ODNs的转染效率；荧光显微镜检测ODNs入核情况；RT-PCR及Western blot检测转染ODNs前后bcl-xl、cyclinD1和c-myc表达水平的变化。结果：ODNs可有效地转染至细胞内,部分可进入核内,转染效率呈剂量依赖关系；STAT3 decoy ODNs可抑制膀胱癌细胞的增殖,100 nmol/L组抑制率最明显,达40%～48%；100 nmol/L decoy ODNs明显抑制bcl-xl、cyclinD1和c-myc mRNA及蛋白的表达水平。结论：STAT3 decoy ODNs可显著抑制膀胱癌细胞的增殖。其作用机制可能是通过下调癌基因c-myc、细胞周期基因cyclinD1及凋亡相关基因bcl-xl的基因转录及蛋白表达而实现的。
AIM: To investigate the inhibitory effect of decoy ODNs on the proliferation of human bladder cancer cell lines and to explore its mechanism. METHODS: STAT3 decoy ODNs were transfected into bladder cancer T24 and 5637 cells using cationic polymer Sofast as transfection reagent. The proliferation of bladder cancer cells was detected by MTT assay. The transfection efficiency of ODNs was detected by flow cytometry. The ODNs were detected by fluorescence microscopy. RT-PCR and Western blot were used to detect the expression of bcl-xl, cyclinD1 and c-myc before and after ODNs transfection. Results: ODNs could be efficiently transfected into the cells and partly into the nucleus, and the transfection efficiency was dose-dependent. STAT3 decoy ODNs could inhibit the proliferation of bladder cancer cells, with the most obvious inhibition rate of 100 nmol / L group reaching 40% ~ 48%; 100 nmol / L decoy ODNs significantly inhibited the expression of bcl-xl, cyclinD1 and c-myc mRNA and protein. Conclusion: STAT3 decoy ODNs can significantly inhibit the proliferation of bladder cancer cells. Its mechanism may be through downregulation of oncogene c-myc, cell cycle gene cyclinD1 and apoptosis-related gene bcl-xl gene transcription and protein expression achieved.