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目的 了解上海地区某动物园灵长类动物隐孢子虫感染情况.方法 2013年3月至2014年10月,采集上海市某动物园灵长类动物的新鲜粪样,经金胺酚-改良抗酸染色后,镜下观察隐孢子虫卵囊.采用巢式PCR扩增18S rRNA基因,测序分析后鉴定虫种,采用Mega7软件用邻接法构建系统进化树.结果 共采集8科37种灵长类动物新鲜粪样83份,镜检未见隐孢子虫卵囊.巢式PCR检测其中3份粪样(6号红猩猩、42号和44号山魈的粪样)隐孢子虫阳性,DNA阳性率为3.61％ (3/83).测序结果显示,与安氏隐孢子虫(Cryptosporidiumandersoni) (GenBank登录号KT175424、KF271479、KF271467)的同源性均为99％.系统进化树显示,3份阳性样品的基因序列与其他来源的安氏隐孢子虫(GenBank登录号KT175424、KF271479、KF271467、KF826314、KJ917578)亲缘关系相近,处于同一分支,表明本次分离的虫株为安氏隐孢子虫.结论 上海地区某动物园灵长类动物存在安氏隐孢子虫感染,具有潜在人兽共患的风险.“,”Objective To determine Cryptospordium infection in primates in a zoo of Shanghai.Methods Fresh fecal samples of primates were collected in a zoo in Shanghai from March 2013 to October 2014.The samples underwent Auramine phenol and modified acid-fast staining to detect oocysts of Cryptosporidium.The 18S rRNA gene was amplified by nested PCR.The PCR products were sequenced and phylogenetic trees were constructed with the neighbor-joining method using Mega 7 software.Results A total of 83 fecal samples were collected from primates from 8 families,37 species.None was positive for oocysts of Cryptospordium.Nested PCR of 18S rRNA gene generated positive products from 3 samples from No.3 orangutan,No.42 and No.44 mandrills,respectively,with a positive rate of 3.61％(3/83).The sequences of the three products all shared 99％ identity with Cryptosporidium andersoni (GenBank Accession No.KT175424,KF271479 and KF271467).The phylogenetic tree showed that the three samples were in the same branch as other species of Cryptosporidium andersoni(GenBank Accession No.KT175424,KF271479,KF271467,KF826314 and KJ917578).This implied that the three Cryptosporidium isolates were C.andersoni.Conclusion Cryptospordium infection exists in primates in the zoo in Shanghai,indicating a potential risk of zoonosis.