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目的:观察大鼠骨髓来源的未成熟树突状细胞(i mDCs)联合西罗莫司(SRL)在诱导大鼠同种异体皮肤移植免疫耐受中的协同作用。方法:以雄性Lewis大鼠为供者、Brown-Norway大鼠为受者,建立大鼠同种异体皮肤移植模型。对照(control)组术前不给予任何干预;未成熟树突状细胞(imDCs)组于术前7天经尾静脉注射供者骨髓来源的未成熟树突状细胞;西罗莫司(SRL)组于术后连续7天经胃管灌注西罗莫司;联合(imDCs+SRL)组于术前7天经尾静脉注射供者骨髓来源的未成熟树突状细胞,并于术后连续7天经胃管灌注西罗莫司。结果:对照组、未成熟树突状细胞(imDC)组、西罗莫司(SRL)组、联合(imDCs+SRL)组大鼠同种异体皮肤移植物术后存活时间分别为(8.25±1.75)(、10.25±1.91)(、10.64±2.50)(、21.38±2.97)天。方差分析提示组间差异有统计学意义(P<0.05);S-N-K检验提示除单独应用未成熟DC组与单独应用SRL组外,各组间的差异均有统计学意义(P<0.05)。结论:供者骨髓来源未成熟树突状细胞可诱导大鼠同种异体皮肤移植免疫耐受;联合使用西罗莫司可延长移植皮片成活时间。
OBJECTIVE: To observe the synergistic effect of immature DCs derived from rat bone marrow (i mDCs) and sirolimus (SRL) on induction of allograft skin allograft tolerance in rats. METHODS: Male Lewis rats were used as donors and Brown-Norway rats as recipients to establish a rat allogenic skin graft model. In the control group, no intervention was given preoperatively. Immature dendritic cells (imDCs) were immature dendritic cells from the donor bone marrow injected via caudal vein 7 days prior to surgery. The rats in group imDCs + SRL were injected with immature DCs derived from bone marrow by tail vein 7 days before the operation, and 7 days after operation. Stromal intratracheal sirolimus infusion. Results: The survival time of control group, imDC group, sirolimus group and imDCs + SRL group were (8.25 ± 1.75 ) (, 10.25 ± 1.91) (, 10.64 ± 2.50) (, 21.38 ± 2.97) days. Analysis of variance showed that there was significant difference between groups (P <0.05). The S-N-K test showed that there was significant difference among all groups except immature DC group and SRL group alone (P <0.05). CONCLUSION: Immature dendritic cells from donor bone marrow can induce immune tolerance of allograft skin allograft in rats. Combined use of sirolimus can prolong survival time of transplanted skin graft.