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目的 :建立头孢唑啉血清、关节腔液浓度测定方法 ,并以此观察其在化脓性膝关节炎或骨性关节炎伴感染患者关节腔液中的分布情况 ,为临床合理用药、提高疗效提供依据。方法 :采用反相高效液相色谱法测定头孢唑啉给药后即刻、0 .5、1、1.5、2、2 .5、3、5、7及 2 4h的血清和关节腔液的药物浓度。给药方法为 70 mg/kg头孢唑啉加入 2 5 0 ml生理盐水中 iv gtt,30min。结果 :头孢唑啉在 2～ 10 0及 10 0～ 5 0 0︼g/ml范围内药物浓度和药物峰面积与内标物峰面积之比线性关系良好。头孢唑啉在血清及关腔液中分布浓度个体差异较大 ,关节腔液中达峰时较血清滞后 1.5 h左右。结论 :本法测定头孢唑啉体液浓度简便可靠 ,能较好地满足临床治疗药物监测的需要
OBJECTIVE: To establish a method for the determination of cefazolin concentration in serum and articular cavity, and to observe the distribution of cefazolin in the articular cavity fluid of patients with purulent knee osteoarthritis or osteoarthritis, so as to provide rational clinical medication and improve curative effect in accordance with. Methods: The concentrations of serum and articular cavity fluid of cefazolin at 0.5, 1, 1.5, 2, 2.5, 3, 5, 7 and 24 h were determined by reversed-phase high performance liquid chromatography . Administration method: 70 mg / kg cefazolin added 250 ml normal saline iv gtt, 30min. Results: The linear relationship between the concentration of cefazolin and the peak area of internal standard was good in the range of 2 ~ 100 and 100 ~ 500 g / ml. Cefazolin in the serum and the concentration of the body cavity distribution of the individual differences are large, the peak in the joint fluid than the serum lag about 1.5 h. Conclusion: The method of determination of cefazolin fluid concentration is simple and reliable, and can better meet the needs of clinical treatment of drug monitoring