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目的快速制备壳聚糖-量子点荧光探针并将其用于细胞标记。方法优化前驱体溶液中的CdCl2、巯基乙酸、KHTe的比例,改变前驱体溶液的pH值和微波加热温度及时间,采用微波加热的方式在水溶液中快速合成CdTe量子点;为提高该CdTe量子点的生物相容性将其与羧甲基壳聚糖通过自组装,制备了壳聚糖-量子点荧光探针,用于狗肾细胞的标记。结果合成的壳聚糖-量子点荧光探针稳定时间超过10个月以上,荧光强度高,量子产率达到75%,半峰宽为42 nm,平均粒径约为3.5 nm,成功地用于了狗肾细胞标记。结论该荧光探针制备简便,荧光性质优异,具有较好的细胞相容性,在生物标记方面具有良好的应用前景。
Aim To rapidly prepare chitosan-quantum dot fluorescence probe and use it for cell labeling. Methods CdCl2, thioglycolic acid and KHTe ratio in the precursor solution were optimized. The precursor solution pH and microwave heating temperature and time were optimized. The CdTe QDs were rapidly synthesized in aqueous solution by microwave heating. Chitosan-QDs were prepared by self-assembly with carboxymethylated chitosan for labeling of canine kidney cells. Results The chitosan-QDs were stable for more than 10 months with high fluorescence intensity, quantum yield of 75%, half-width of 42 nm and average diameter of 3.5 nm. The dog kidney cell markers. Conclusion The fluorescent probe has the advantages of simple preparation, excellent fluorescence property, good cell compatibility and good application prospect in biomarker.