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离体肾脏灌流(IPK)是应用比较早的离体器官灌流实验方法,具有去除神经和体液等影响、便于施加干扰因素以及实时监测肾脏功能变化的特点,可用于研究药物肾脏代谢与排泄及其机制等。本文通过对国外利用IPK技术进行研究的文献进行综述,旨在拓展国内利用IPK技术进行研究的视角。通过整理、归纳、分析大量国外利用IPK技术进行研究的文献,本文主要从肾小球滤过调节机制、肾小管和集合管重吸收和分泌调节机制以及肾脏局部缺血损伤模型等多个方面探讨IPK技术在研究肾脏分子水平上的变化以及肾脏发病机制和肾病发生发展过程中各调节因素相互作用等方面的应用。通过大量文献的分析,发现国外运用IPK技术比较成熟,而国内运用此技术主要研究药物的代谢排泄机制、药物性肾损伤机制或供体肾脏移植等方面,较为局限。本文针对IPK技术在肾脏分子水平上的变化以及肾脏发病机制等研究中的应用进行介绍,可以为国内学者利用IPK技术在研究药物以及致病因素刺激下肾脏功能改变及其机制等方面提供参考,并指出IPK技术在整体肾脏角度研究足细胞形态结构和功能改变及其分子机制方面有重要意义。
In vitro renal perfusion (IPK) is an earlier application of isolated organ perfusion experiments, with the removal of nerve and body fluids, to facilitate the application of interference factors and real-time monitoring of renal function changes can be used to study drug metabolism and excretion of kidney Mechanism and so on. This article summarizes the literature on IPK technology research abroad, and aims to expand the domestic research perspective of using IPK technology. Through the collation, induction and analysis of a large number of foreign literature using IPK technology, this article mainly from the aspects of glomerular filtration regulation mechanism, renal tubular and collecting tube reabsorption and secretion regulation mechanism and renal ischemia injury model and other aspects IPK technology in the study of changes in the level of renal molecules and kidney pathogenesis and development of renal disease in the regulation of the interaction between the factors such as the application. Through a large number of literature analysis, it is found that IPK technology abroad is relatively mature. However, the domestic application of this technology mainly focuses on the mechanism of drug excretion, drug-induced renal injury or donor kidney transplantation. In this paper, IPK technology in renal changes in the molecular level and the pathogenesis of renal applications such as research are introduced for domestic scholars using IPK technology in the study of drugs and pathogenic factors to stimulate renal function changes and its mechanisms provide a reference, And pointed out that IPK technology in the perspective of the whole kidney podocyte morphology and function changes and its molecular mechanisms of great significance.