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目的通过检测乳腺癌患者外周血细胞角蛋白19(CK19)mRNA,研究外周血微转移情况与肿瘤大小、腋淋巴结转移情况、临床分期、年龄、绝经与否及受体情况有无相关性。方法以60例乳腺癌术后患者为研究对象,根据临床分期分为三组(0～Ⅰ期、Ⅱ期、Ⅲ期),运用RT-PCR技术,以CK19mRNA为标志物,分别检测其血液微转移发生情况。结果血液微转移阳性率为23.33%(14/60),其中0～Ⅰ期0.00%(0/9)、Ⅱ期16.22%(6/37)、Ⅲ期57.14%(8/14),三组之间差异有统计学意义(P<0.05)。结论血液微转移与肿瘤分期密切相关。血液微转移与肿瘤大小、腋淋巴结转移状态、年龄、绝经与否及受体情况无相关性。
Objective To detect the expression of cytokeratin 19 (CK19) mRNA in peripheral blood of patients with breast cancer, and to investigate the relationship between micrometastasis in peripheral blood and tumor size, axillary lymph node metastasis, clinical stage, age, menopause and receptor status. Methods Sixty patients with postoperative breast cancer were enrolled in this study. The patients were divided into three groups (stage 0 to stage I, stage II and stage III) according to clinical stage. RT-PCR and CK19 mRNA were used as markers to detect blood micro-level Transfer occurred. Results The positive rate of blood micrometastasis was 23.33% (14/60), of which 0.00% (0/9) in stage 0 to stage Ⅰ, 16.22% (6/37) in stage Ⅱ, 57.14% (8/14) in stage Ⅲ, The difference was statistically significant (P <0.05). Conclusions Blood micrometastasis is closely related to tumor stage. There was no correlation between blood micrometastasis and tumor size, status of axillary lymph node metastasis, age, menopause and receptor status.