论文部分内容阅读
从瓜叶菊中克隆了10个谷胱甘肽转移酶(GST)基因,命名为ScGST1~10。序列相似性比较与基因表达分析结果显示,ScGST3可能是一个花青素苷转运相关的候选基因。对RT-PCR扩增的ScGST3序列分析发现,其包含一个639bp的开放阅读框,编码212个氨基酸残基,含有3个外显子和2个内含子,属于phi型GST;氨基酸序列比较分析表明,其与仙客来(Cyclamen persicum)CkmGST3、香石竹(Dianthus caryophyllus)DcGSTF2和矮牵牛(Petunia hybrida)PhAN9等花青素苷转运相关的GST具有较高的相似性;荧光定量PCR分析表明,该基因在含有花青素苷的组织中表达信号较强,在花序发育早期表达丰度最高,花序开放末期表达量下降。据此推测分离得到的ScGST3可能与瓜叶菊中花青素苷的转运与积累相关。
Ten glutathione transferase (GST) genes were cloned from cineraria and named ScGST1 ~ 10. Sequence similarity analysis and gene expression analysis showed that ScGST3 may be a candidate gene related to anthocyanin transport. Analysis of the ScGST3 sequence amplified by RT-PCR revealed that it contained a 639 bp open reading frame, encoding 212 amino acid residues, containing 3 exons and 2 introns, which belonged to phi type GST. Amino acid sequence comparison analysis The results showed that GST had high similarity with GST related to the translocation of anthocyanins such as Cyclamen persicum CkmGST3, Dianthus caryophyllus DcGSTF2 and Petunia hybrida PhAN9. Fluorescence quantitative PCR analysis showed that , Which expressed strongly in the tissues containing anthocyanin, had the highest expression in the early stages of inflorescence and decreased in the late stage of inflorescence. It is speculated that the isolated ScGST3 may be related to the transport and accumulation of anthocyanins in the cineraria.