【摘 要】
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目的 观察灯盏乙素(Scu)对β淀粉样蛋白(Aβ1-42)诱导的SH-SY5Y细胞模型中N-甲基-D-天门冬氨酸受体亚型1(NMDAR1)的影响.方法 将神经母细胞瘤(SH-SY5Y)细胞分为对照组、Aβ寡聚体处理组、Scu处理组和Scu+Aβ寡聚体处理组,采用蛋白印迹法检测各组细胞中NMDAR1的蛋白表达情况,实时荧光定量PCR检测NMDAR1 mRNA表达水平,荧光探针法测定各组细胞氧自由基(ROS)的水平,黄嘌呤氧化酶法测定各组细胞超氧化物歧化酶(T-SOD)的活力.结果 与对照组比较,Scu处理组
【机 构】
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贵州医科大学附属医院 病理科,贵州 贵阳 550004;贵阳市第一人民医院 病理科,贵州 贵阳 550002
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目的 观察灯盏乙素(Scu)对β淀粉样蛋白(Aβ1-42)诱导的SH-SY5Y细胞模型中N-甲基-D-天门冬氨酸受体亚型1(NMDAR1)的影响.方法 将神经母细胞瘤(SH-SY5Y)细胞分为对照组、Aβ寡聚体处理组、Scu处理组和Scu+Aβ寡聚体处理组,采用蛋白印迹法检测各组细胞中NMDAR1的蛋白表达情况,实时荧光定量PCR检测NMDAR1 mRNA表达水平,荧光探针法测定各组细胞氧自由基(ROS)的水平,黄嘌呤氧化酶法测定各组细胞超氧化物歧化酶(T-SOD)的活力.结果 与对照组比较,Scu处理组NMDAR1蛋白、NMDAR1 mRNA表达、ROS含量及T-SOD含量比较,差异无统计学意义(P>0.05);Aβ寡聚体处理组细胞NMDAR1蛋白和NMDAR1 mRNA表达水平升高、细胞ROS含量升高、细胞T-SOD含量降低,差异均有统计学意义(P<0.05);与Aβ寡聚体处理组比较,Scu+Aβ寡聚体处理组NMDAR1蛋白和NMDAR1 mRNA表达水平下调、细胞ROS含量降低、细胞T-SOD含量升高,差异均有统计学意义(P<0.05).结论 Scu可能通过下调NMDAR1 mRNA、减少NMDAR1蛋白表达,从而对抗Aβ寡聚体的毒性.
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