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对植物组织中蚜虫口针分泌的胶状唾液染色观察发现,蚜虫刺探时口针穿行在细胞间隙,仅在吸食时下颚口针进入细胞内。禾谷缢蚜饲毒12h时RT-PCR方法在后肠、血淋巴液和附唾液腺中检测到GPV;而非介体麦长管蚜在GPV毒源上吸食24h时,仅在后肠组织中检测到GPV;当继续取食48h时,在后肠及血淋巴液中也发现了GPV,但仍无能力进入附唾液腺,说明蚜虫后肠和附唾液腺对病毒均有专化选择作用,禾谷缢管蚜传播BYDV-GPV时存在吸食、获毒和传毒三个阶段。
On the aphid stitches saliva stained by the stitches of the aphids in plant tissues, it was found that when the aphids were stabbed, the stitches traveled through the interstitial spaces, and the jaws only entered the cells during feeding. GPV was detected in the hindgut, hemolymph and salivary glands by RT-PCR at 12h after the feeding of H. armigera, while GPV was detected only in the hindgut tissues GPV was detected; GPV was also found in the hindgut and hemolymph when feeding for 48h, but still unable to enter the salivary gland, indicating that the aphids hindgut and salivary gland had special selective effects on the virus, Aphid spread BYDV-GPV there are three stages of smoking, getting poison and poisoning.