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[目的]利用AGGM标记对甘蓝型油菜大籽粒材料进行聚类分析。[方法]根据18个拟南芥籽粒发育相关基因,并结合同源油菜EST序列,设计开发44对ACGM引物。选择其中18对引物对16份大籽粒材料和4份小籽粒材料进行聚类分析。[结果]这18对引物分别稳定扩增出2-6条特异条带,扩增差异明显,多态性丰富,重复度高,表明优化的ACGM标记在甘蓝型油菜中具有良好的通用性。聚类结果显示20份材料在相似系数0.6处分为A、B、C3个类群,其中A、B类又可在相似系数0.67处分为A1、A2、A3、B1、B2五个亚群。[结论]为下一步利用这些材料进行育种及相关科研工作提供一定的理论基础。
[Objective] The research aimed to cluster the large grain materials of Brassica napus with AGGM marker. [Method] According to 18 ESTs related to Arabidopsis thaliana seed development and combining with homologous rape EST sequences, 44 pairs of ACGM primers were designed and developed. 18 pairs of primers were selected for cluster analysis of 16 large grain materials and 4 small grain materials. [Result] The 18 pairs of primers could stably amplify 2-6 specific bands respectively. The amplification showed obvious difference, rich polymorphism and high repeatability, indicating that the optimized ACGM marker has good versatility in Brassica napus. The clustering results showed that the 20 materials were divided into A, B and C3 groups at a similarity coefficient of 0.6. Among them, A and B groups could be divided into A1, A2, A3, B1 and B2 subgroups at a similarity coefficient of 0.67. [Conclusion] This provided some theoretical basis for the future use of these materials for breeding and related research work.