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本文报道用硅胶G—CMC薄层层析,以石油醚(30～60℃)—乙酸乙酯(95:5)为展开剂,将莪术油中莪术醇与其它成分分离,用10％香草醛冰醋酸(5:1)显色,岛津CS—910双波长薄层扫描仪进行含量测定。测定采用反射法、直线扫描,测定波长λs=515nm、λR=730nm,结果由数字显示器显示。本法具有快速、准确、简便、分离效果好和微量等优点。样品显色后两小时内积分值相对稳定,取样1～7μl斑点浓度和积分值呈线性关系,回归方程y=8.30+60.97x,相关系数0.9953。含1μl以上莪术醇就可以定量,平均回收率为103.3％,同一块板上不同斑点变异系数<4.5％,同一批号不同板之间无显著性差异。
This article reports the use of silica gel G-CMC thin layer chromatography, petroleum ether (30 ~ 60 °C) - ethyl acetate (95:5) as a developing agent, the Curcuma oil in Curcuma oil and other components, with 10% vanillin Glacial acetic acid (5:1) was developed and a Shimadzu CS-910 dual-wavelength thin-layer scanner was used for content determination. The measurement was performed by a reflection method and a linear scan. The measurement wavelength λ s = 515 nm and λ R = 730 nm, and the results were displayed on a digital display. This method has the advantages of rapidity, accuracy, simplicity, good separation effect and trace amount. The integral value was relatively stable within two hours after the coloration of the sample. The linear relationship between the spot concentration and the integral value of sampling was from 1 to 7 μl. The regression equation was y=8.30+60.97x and the correlation coefficient was 0.9953. Containing more than 1 μl Curcumol can be quantified with an average recovery of 103.3%. The variation coefficient of different spots on the same plate is <4.5%. There is no significant difference between different plates of the same batch number.