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采用营养液砂培方法,研究外源一氧化氮(NO)供体硝普钠(SNP)对NaHCO3胁迫下黑麦草幼苗生长、活性氧代谢和渗透溶质积累的影响。结果表明:60μmol·L-1SNP能够缓解100mmol·L-1NaHCO3胁迫对黑麦草幼苗生长的抑制作用,减缓NaHCO3胁迫导致的叶片O2·-产生速率、H2O2和丙二醛(MDA)含量的增加,提高NaHCO3胁迫下幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、质膜H+-ATP酶的活性和谷胱甘肽(GSH)、可溶性糖、可溶性蛋白质和脯氨酸的含量及K+/Na+比,降低过氧化氢酶(CAT)活性和抗坏血酸(AsA)含量,对游离氨基酸含量影响不大。上述结果表明,NO可能通过激活抗氧化系统活性、促进渗透溶质积累和改善Na+、K+平衡等缓解碱胁迫对幼苗的伤害,从而提高黑麦草的耐碱性。
The effects of exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) on the growth, active oxygen metabolism and osmotic solute accumulation of ryegrass seedlings under NaHCO3 stress were studied by nutrient sands culture. The results showed that 60μmol·L-1 SNP can alleviate the inhibition of 100 mmol·L-1 NaHCO3 stress on the growth of ryegrass seedlings, and alleviate the increase of O2 · - production rate, H2O2 and malondialdehyde (MDA) content induced by NaHCO3 stress The activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), plasma membrane H + -ATPase and the activities of glutathione (GSH), soluble sugar , Soluble protein and proline content and K + / Na + ratio decreased CAT activity and ascorbic acid (AsA) content, but had little effect on the content of free amino acids. The above results show that NO may improve the alkali resistance of ryegrass by activating the activity of antioxidant system, promoting the accumulation of osmotic solutes and improving the balance of Na +, K + to alleviate the damage to seedlings caused by alkali stress.