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目的评价硒多糖对小鼠免疫功能的影响。方法 SPF级雄性BALB/c小鼠随机分为对照组、硒多糖低、中、高剂量组(0.13g/kg体质量、0.27g/kg体质量、0.40g/kg体质量),按分组灌胃硒多糖30d。测定主要免疫器官(胸腺、脾)脏器系数;并检测小鼠细胞免疫功能指标(脾淋巴细胞转化能力、迟发型变态反应)、体液免疫功能指标(血清溶血素凝集水平、溶血空斑生成)、非特异性免疫功能指标(吞噬细胞吞噬鸡红细胞能力、吞噬细胞碳廓清能力、NK细胞活性)。结果中剂量组胸腺系数高于对照组(P<0.05);血清溶血素凝集试验示高剂量组血清抗体积数高于对照组(P<0.05);中、高剂量组溶血空斑数高于对照组(P<0.05);低、中剂量组NK细胞活性高于对照组,而在该剂量范围内对脾脏系数、脾淋巴细胞转化能力、迟发型变态反应、吞噬细胞吞噬鸡红细胞能力、吞噬细胞碳廓清能力未表现出明显影响(P>0.05)。结论硒多糖对免疫功能可能有一定影响,仍需进一步研究。
Objective To evaluate the effect of selenium polysaccharide on immune function in mice. Methods SPF male BALB / c mice were randomly divided into control group, low, medium and high dose selenium group (0.13g / kg body weight, 0.27g / kg body weight, 0.40g / kg body weight) Stomach selenium polysaccharide 30d. The organ function of major immune organs (thymus and spleen) was measured. The indexes of cellular immune function (such as splenic lymphocyte transformation ability and delayed-type allergy), indexes of humoral immunity (serum hemolysin level and hemolytic plaque formation) , Non-specific immune function (phagocytosis of chicken erythrocytes, phagocytic carbon clearance ability, NK cell activity). Results The thymus coefficient of the middle dose group was higher than that of the control group (P <0.05). The serum hemolysin agglutination test showed that the anti-tumor volume of the high dose group was higher than that of the control group (P <0.05) (P <0.05). The NK cell activity in low and middle dose groups was higher than that in control group, while the spleen coefficient, splenic lymphocyte transformation ability, delayed-type allergic reaction, phagocytosis of chicken erythrocytes, phagocytosis Cell carbon clearance ability showed no significant effect (P> 0.05). Conclusion Selenium polysaccharide may have some influence on immune function, but further study is needed.