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目的 研究芥子气 (MG)对大鼠骨髓细胞DNA的损伤作用。方法 雄性SD大鼠随机分为 6组 ,腹腔注射生理盐水 (NS)、丙二醇、MG(0 .2、0 .4、0 .8、1.6mg/kg体重 ) ,分别于染毒后 0、2 4、4 8、72h处死各组的 5只大鼠 ,用单细胞凝胶电泳法分析大鼠骨髓细胞DNA的损伤情况。结果 在染毒后 0h各组的大鼠骨髓细胞DNA损伤差异无显著性 (P >0 .0 5 ) ;丙二醇组的大鼠骨髓细胞DNA迁移率和迁移度在染毒后 2 4、4 8、72h分别为 15 .4 %± 0 .2 1%、16 .0 %± 0 .19%、15 .7%± 0 .2 3%和 (11.4±0 .2 )、(13.5± 0 .3)、(12 .8± 0 .2 ) μm ,明显高于在同时刻的NS组水平 ,差异有显著性 (P <0 .0 5 ) ;各MG组的大鼠骨髓细胞DNA迁移率和迁移度在染毒后 2 4、4 8、72h分别高于在同时刻的NS组和丙二醇组水平 ,差异有显著性 (P <0 .0 5 )。结论 MG对大鼠骨髓细胞DNA有损伤作用 ,随剂量的增大损伤有上升的趋势 ,损伤呈时间依赖性。
Objective To study the effect of mustard gas (MG) on the DNA damage of rat bone marrow cells. Methods Male Sprague-Dawley rats were randomly divided into 6 groups: intraperitoneal injection of normal saline (NS), propanediol, MG (0.2, 0.4, 0.8, 1.6 mg / kg body weight) On the 4th, 8th and 72th hours, 5 rats in each group were sacrificed and the DNA damage in rat bone marrow cells was analyzed by single cell gel electrophoresis. Results There was no significant difference in the DNA damage of rat bone marrow cells between groups at 0h (P> 0.05). The DNA mobility and migration of the rat bone marrow cells in propylene glycol group were significantly lower than those of the control group And 72h were 15.4% ± 0.21%, 16.0% ± 0.19%, 15.7% ± 0.233% and (11.4 ± 0.2%), (13.5 ± 0.3% ), (12.8 ± 0.2) μm, which was significantly higher than that of the NS group at the same time point (P <0.05). The DNA mobility and migration of rat bone marrow cells in each MG group The degrees were significantly higher at 2, 4, 8 and 72 hours after exposure than those at the same time points in the NS and propanediol groups, respectively (P <0.05). Conclusion MG can injure the DNA of rat bone marrow cells. The injury tends to increase with dose increasing, and the damage is time-dependent.