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目的 研究端粒酶活性与胃癌细胞生物学行为的相关性,评估端粒酶活性检测的临床价值。方法 通过非放射性的端粒重复扩增法(TRAP)分析和银染技术测定47例胃癌组织和癌旁组织、7例胃溃疡组织和2种胃癌细胞株中的端粒酶活性。结果 47例癌旁组织均无端粒酶活性,41例胃癌组织中均检测到端粒酶活性,阳性率87.2%(P<0.001)。2例胃癌细胞株也检测到端粒酶活性,7例胃溃疡组织均为阴性。结论 端粒酶活性与胃癌的生物学行为以及胃组织潜在的恶性病变有一定相关性。端粒酶活性有可能作为胃癌诊断的一种检测指标。
Objective To study the relationship between telomerase activity and biological behavior of gastric cancer cells and to evaluate the clinical value of telomerase activity. Methods Telomerase activity was determined in 47 gastric cancer tissues and adjacent non-cancerous tissues, 7 gastric ulcer tissues and 2 gastric cancer cell lines by non-radioactive telomeric repeat amplification assay (TRAP) and silver staining. Results No telomerase activity was found in 47 cases of paracancerous tissues, and telomerase activity was detected in 41 cases of gastric carcinoma, the positive rate was 87.2% (P <0.001). Telomerase activity was also detected in 2 gastric cancer cell lines, and 7 gastric ulcer tissues were negative. Conclusion The telomerase activity has some correlation with the biological behavior of gastric cancer and the potential malignant lesions of gastric tissue. Telomerase activity may be used as a diagnostic indicator of gastric cancer.