论文部分内容阅读
目的:通过肥甘饮食诱导ApoE-/-小鼠引发高脂血症及对血脂及超氧化物歧化酶、丙二醛影响的研究,探讨高脂血症的伏痰病因学本质。方法:将ApoE-/-小鼠用随机数字表法分为2组,同样将C57BL/6J小鼠分为2组,共4组,每组10只。分别以正常饲料、肥甘饲料喂养。通过生化检测,比较各组小鼠血清中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)的水平及超氧化物歧化酶、丙二醛的变化。结果:喂养8周后,与A组小鼠相比较,B、C、D各组小鼠在TC、TG、HDL-C、LDL-C改善方面有统计学意义(P<0.05);与B、C组比较,D组小鼠在TC、TG、LDL-C改善方面具有统计学意义(P<0.01),而在HDL-C方面,D组与B组比较,有统计学意义(P<0.05),与C组比较,无统计学意义。结论:在饮食干预和(或)先天禀赋不足情况下,血清超氧化物歧化酶活性下降,血清丙二醛含量增高。
Objective: To study the etiology of hyperlipidemia caused by hyperlipidemia by inducing hyperlipidemia and its effects on blood lipid, superoxide dismutase and malondialdehyde in ApoE - / - mice induced by fat diet. Methods: ApoE - / - mice were divided into two groups by random number table. The same C57BL / 6J mice were divided into two groups, a total of 4 groups of 10 mice. Respectively, normal feed, fat feed feeding. The levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and superoxide dismutase and malondialdehyde in serum of the mice in each group were compared by biochemical tests. Results: Compared with group A, the mice in groups B, C and D had significant improvement in TC, TG, HDL-C and LDL-C after 8 weeks of feeding (P <0.05) (P <0.01). Compared with group C, group D had statistical significance in improving TC, TG and LDL-C (P <0.01), while in group HDL-C, there was a significant difference between group D and group B (P < 0.05), compared with the C group, no statistical significance. CONCLUSIONS: Serum superoxide dismutase activity decreased and serum malondialdehyde content increased under dietary intervention and / or deficient innate endowment.