论文部分内容阅读
以蒙古冰草为材料,在离体培养条件下对其幼胚的发育进行了研究。结果表明:蒙古冰草的幼胚在不含任何激素的培养基上能直接萌发,幼胚发芽率因培养基而异。N6+7%蔗糖培养基萌发率最高,达96%;其次为MS+5%蔗糖培养基,萌发率为90%。当幼胚被培养在减半的N6培养基或减半的MS培养基上时,发芽率均显著降低。以上四种培养基均未发生脱分化现象。相反,在含有2,4-二氯苯氧乙酸(2,4-D)的培养基上,蒙古冰草幼胚不同程度地发生了脱分化,并出现了少量再生芽。在继代培养基中,降低2,4-D浓度,附加低浓度6-苄氨基嘌呤(BAP)可以改善蒙古冰草幼胚愈伤组织状态,增加胚性愈伤组织诱导率,从而提高分化率。
The growth of immature embryos was studied under the condition of in vitro culture with the material of M.grass. The results showed that the immature embryos could directly germinate on the medium without any hormones, and the germination rate of immature embryos varied with the culture medium. N6 + 7% sucrose medium had the highest germination rate, reaching 96%; followed by MS + 5% sucrose medium, the germination rate was 90%. Germination rates were significantly reduced when immature embryos were cultured on halved N6 medium or halved MS medium. No dedifferentiation occurred in the above four media. In contrast, on the medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), L. immature embryo dedifferentiated to varying degrees and a small amount of regenerated shoots appeared. In the subculture medium, reducing the concentration of 2,4-D, adding low concentrations of 6-benzylaminopurine (BAP) can improve the Mongolian grass Iris embryo callus status, increased embryogenic callus induction rate, thereby enhancing differentiation rate.