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目的探讨热疗增强顺铂（ＤＤＰ）对子宫颈癌细胞的作用。方法以体外培养的子宫颈癌Ｈｅｌａ细胞株为研究对象，采用组内分组设计方法，以恒温水浴热疗，藉ＭＴＴ法观测不同热疗温度、时间、序贯对ＤＤＰ化疗增效作用。结果（１）Ｈｅｌａ细胞在３７℃下与０．１ｍｇ·Ｌ－１ＤＤＰ作用２４ｈ，细胞毒指数（ＣＩ）为０．０８，辅以４３℃热疗３０ｍｉｎ，ＣＩ值提高８．５倍；而ＤＤＰ浓度提高１０００倍时，ＣＩ值仅提高１０倍。４３℃热疗组与４１℃以下热疗组间的ＣＩ值差异有显著性（Ｐ＜０．０１）。（２）热疗时间１５ｍｉｎ以上组与７．５ｍｉｎ组之间的ＣＩ值差异有显著性（Ｐ＜０．０１），而１５ｍｉｎ以上各组间ＣＩ值差异无显著性（Ｐ＞０．０５）。（３）热疗与ＤＤＰ同时应用者细胞毒性作用最强。结论热疗增强ＤＤＰ对Ｈｅｌａ细胞的毒性作用，以４３℃１５ｍｉｎ以上热疗与ＤＤＰ同时应用效果最佳。
Objective To investigate the effect of hyperthermia and cisplatin (DDP) on cervical cancer cells. Methods In vitro cultured cervical cancer Hela cell line was used as research object. Intragroup grouping design method was used to heat therapy with constant temperature water bath. MTT method was used to observe the synergistic effect of different hyperthermia temperature, time and sequential chemotherapy on DDP. Results (1) Hela cells were treated with 0.1 mg·L-1 DDP at 37°C for 24 h, the cytotoxicity index (CI) was 0.08, and the hyperthermia at 43°C for 30 min increased the CI value by 8.5 times. When the concentration is increased by 1000 times, the CI value only increases by 10 times. There was significant difference in CI values between the 43°C hyperthermia group and the hyperthermia group below 41°C (P<0.01). (2) There was a significant difference in the CI value between the group with 7.5 min and the group with hyperthermia for 15 min (P<0.01), but there was no significant difference between the groups in the CI value between 15 min and above (P>0.05). . (3) Patients with hyperthermia combined with DDP had the strongest cytotoxicity. Conclusion Hyperthermia enhances the toxic effect of DDP on Hela cells. The effect of hyperthermia with DDP at 43°C for 15 min is best.