AIM TO study the difference of gene expression betweenesophageal carcinoma and its pericancerous epithelium andto screen novel associated genes in the early stage ofesophageal carcinogenesis by cDNA microarray.METHODS:Total RNA was extracted with the original singlestep way from esophageal carcinoma,its pericancerousepithelial tissue and normal esophageal epithelium far fromthe tumor.The cDNA retro-transcribed from equal quantityof mRNA was labeled with Cy5 and Cy3 fluorescencefunctioning as probes.The mixed probes were hybridizedwith two pieces of BioDoor 4 096 double dot human wholegene chip.Fluorescence signals were scanned by ScanArray3 000 laser scanner and farther analyzed by ImaGene 3.0software with the digital computer.RESULTS:(1)A total of 135 genes were screened out,inwhich 85 and 50 genes whose the gene expression levels(fluorescence intensity)in esophageal cardnoma were morethan 2 times and less than 0.5 times respectively comparedwith the normal esophageal epithelium.(2)There were alsototal 31 genes,among then 27 and 4 whose expressions inpericancerous tissue were 2-fold up-regulated and 0,5-folddown-regulated respectively compared with normalesophageal epithelium.(3)There were 13 genes appearedsimultaneously in both pericancerous epithelium andesophageal carcinoma,while another 18 genes existed inpericancerous epithelium only.CONCLUSION:With the parallel comparison among thesethree gene profiles,it was shown that(1).A total of 135genes,Whose expression difference manifested asfluorescence intensity were more than 2 times betweenesophageal carcinoma and normal esophageal epithelium,were probably related to the occurrence and developmentof the esophageal carcinoma.(2).The 31 genes showingexpression difference more than 2 times betweenpericancerous and normal esophageal epithelium might berelate to the promotion of esophageal pericancerosis andits progress.The present study illustrated that by using thegene chip to detect the difference of gene expression profiles might be of benefit to the gene diagnosis,treatment andprevention of esophageal carcinoma. AIM TO study the difference of gene expression betweenesophageal carcinoma and its pericancerous epithelium and to screen novel associated genes in the early stage of esophageal carcinogenesis by cDNA microarray. METHODS: Total RNA was extracted with the original singlestep way from esophageal carcinoma, its pericancerousepithelial tissue and normal esophageal epithelium far from the tumor. The cDNA was retro-transcribed from equal quantityof mRNA was labeled with Cy5 and Cy3 fluorescencefunctioning as probes.The mixed probes were hybridizedwith two pieces of BioDoor 4 096 double dot human wholegene chip. Fluorescence signals were scanned by ScanArray3 000 laser scanner (1) A total of 135 genes were screened out, inwhich 85 and 50 genes whose the gene expression levels (fluorescence intensity) in esophageal cardnoma were more than 2 times and less than 0.5 times respectively compared with the normal esophageal epithelium. (2) Th ere were alsototal 31 genes, among then 27 and 4 whose expressions in pericancerous tissue were 2-fold up-regulated and 0,5-fold down-regulated respectively compared with normalesophageal epithelium. (3) There were 13 genes appeared both surely in both pericancerous epithelium andesophageal carcinoma , while another 18 genes existed in pericancerous epithelium only. CONCLUSION: With the parallel comparison among these three gene profiles, it was shown that (1). A total of 135 genes, Whose expression difference manifested as fluorescence intensity were more than 2 times betweenesophageal carcinoma and normal esophageal epithelium, were probably related to the occurrence and development of the esophageal carcinoma. (2). The 31 genes showingexpression difference more than 2 times between pericancerous and normal esophageal epithelium might berelate to the promotion of esophageal pericancerosis andits progress.The present study shows that by using thegene chip to detect the difference of gene expressionprofiles might be of benefit to the gene diagnosis, treatment and prevention of esophageal carcinoma.