论文部分内容阅读
作者观察了亚硝基胍(MNNG)、丝裂霉素(MMC)和乙基磺酸甲酯(EMS)对V79细胞及其γ线敏感的突变株irs5和irs11存活率和染色体畸变的影响。存活率测定的药物浓度MNNG为2.5～30.0μmol/L,MMC为1～20μmol/L,EMS为2～16mmol/L;irs5的存活率和细胞克隆形成数均优于V79,而irs11明显低于前两者。染色体畸变测定IC_(50)的结果与存活率相似。irs5和V79由MNNG,MMC和EMS引起的染色体畸变,其类型和数量相似,染色体畸变率均呈剂量-反应关系,且自发畸变率<4％。irs11的畸变率未见明显的剂量反应,自发畸变率>10％。结果提示irs5细胞生物学特性较为稳定,进一步研究可确定其是否可作为新型的突变细胞株用于致突性或致癌性研究。
The authors observed the effects of nitrosoguanidine (MNNG), mitomycin (MMC) and methyl ethyl methanesulfonate (EMS) on the survival and chromosomal aberrations of VRS cells and their γ-sensitive mutants, irs5 and irs11. Survival rate of MNNG was 2.5 ~ 30.0μmol / L, MMC was 1 ~ 20μmol / L, EMS was 2 ~ 16mmol / L; irs5 survival rate and cell clone formation number were better than V79, and irs11 was significantly lower than The first two. Chromosome aberration assay IC_ (50) results and survival rate similar. The chromosome aberrations induced by MNNG, MMC and EMS were similar in irs5 and V79. The types and numbers of chromosomal aberrations were in a dose-response relationship with spontaneous aberrations of <4%. The distortion rate of irs11 no obvious dose response, spontaneous distortion rate> 10%. The results suggest that the biological characteristics of irs5 cells more stable, further research to determine whether it can be used as a new mutant cell lines for the study of the sudden or carcinogenicity.