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目的:研究青白散抗炎作用及其机理。方法:采用小鼠二甲苯性耳肿胀,大鼠蛋清性足肿胀和棉球性肉芽肿等多种炎症模型,观察本品抗炎作用;采用慢性软组织损伤动物模型,检测大鼠慢性软组织损伤后组织中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)的表达,并分析其相关性。结果:(1)青白散可明显抑制小鼠二甲苯性耳肿胀,显著抑制大鼠蛋清性足肿胀和棉球性肉芽组织增生。(2)青白散能显著抑制大鼠慢性软组织损伤后组织中致炎因子TNF-α和IL-6表达的升高差异。(3)青白散能显著降低大鼠慢性软组织损伤后肌组织中NO含量和iNOS的活性。结论:青白散有明显的抗炎作用,可能通过抑制大鼠慢性损伤组织中致炎因子TNF-α和IL-6的含量增高,抑制iNOS诱导过量NO的产生,为慢性软组织损伤的修复创造了有利条件。
Objective: To study the anti-inflammatory effect and its mechanism of Qingbai San. Methods: Anti-inflammatory effects were observed in mice with xylene-induced ear swelling, rat paw edema, and granulomatous granuloma in rats. The chronic soft tissue injury model was used to detect the chronic soft tissue injury (TNF-α), interleukin 6 (IL-6), nitric oxide (NO) and inducible nitric oxide synthase (iNOS) were detected by immunohistochemistry and their correlations were analyzed. Results: (1) Qingbai San can significantly inhibit xylene ear swelling in mice, significantly inhibited rat egg white paw swelling and granulosa granulation tissue hyperplasia. (2) Qingbai San can significantly inhibit the expression of TNF-α and IL-6 in the tissue after chronic soft tissue injury in rats. (3) Qingbai San can significantly reduce NO content and iNOS activity in muscle tissue after chronic soft tissue injury in rats. CONCLUSION: Qingbai San has obvious anti-inflammatory effect, which may create an effect of repairing chronic soft tissue injury by inhibiting the increase of inflammatory cytokines TNF-α and IL-6 and inhibiting the excessive nitric oxide induced by iNOS in rats Favorable conditions