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抗氧化肽是蛹虫草的重要活性成分之一。以蚕蛹虫草子实体制备的纳米蛹虫草粉为基质,利用地衣芽孢杆菌(Bacillus licheniformis)E-30菌株进行液态发酵,在接种量为3%、碳氮比0.4、发酵基质质量浓度25 g/L的条件下,蛹虫草初级抗氧化肽的得率为6.4 g/L。体外试验检测该抗氧化肽在质量浓度为1 mg/m L时,对DPPH、·OH以及O-2的清除率分别为68.12%、79.40%和82.30%。辐射氧化损伤(60Co-γ照射总剂量8 Gy)模型小鼠按80 mg/(kg·d)的剂量灌胃蛹虫草抗氧化肽3 d后,小鼠血液中的白细胞、淋巴细胞含量均显著增加(P<0.01);灌胃10 d后,小鼠血液中的白细胞、淋巴细胞含量趋于正常水平,超氧化物歧化酶活性上升,丙二醛含量降低。采用DEAE-52、Sephadex G-25对制备的蛹虫草抗氧化肽逐级纯化,得到的抗氧化肽单峰经质谱检测含有分子质量为2 237 D、3 372 D的2个组分,是富含芳香族氨基酸和碱性氨基酸的2个寡肽的混合物。研究结果提示,蛹虫草抗氧化肽对小鼠因辐射引起的血象变化及氧化损伤具有修复作用。
Antioxidant peptides are one of the important active ingredients of Cordyceps militaris. Using Cordyceps militaris powder prepared from silkworm Cordyceps militaris as substrate, Bacillus licheniformis strain E-30 was used for liquid fermentation. Under inoculation amount of 3%, C / N ratio of 0.4, fermentation medium concentration of 25 g / L , The yield of primary antioxidant peptide of Cordyceps Militaris was 6.4 g / L. In vitro tests showed that the anti-oxidative peptides scavenging DPPH, · OH and O-2 were 68.12%, 79.40% and 82.30% at the concentration of 1 mg / mL. Radiation oxidative injury (60Co-γ irradiation, a total dose of 8 Gy) mice at 80 mg / (kg · d) dose of oral Cordyceps militaris antioxidant peptide 3 d, the mice blood leukocytes, lymphocytes were significantly (P <0.01). The levels of leukocyte and lymphocyte in the blood of the mice tended to be normal after 10 days of gavage, the activity of superoxide dismutase increased and the content of malondialdehyde decreased. The purified anti-oxidation peptide of Cordyceps militaris was purified by DEAE-52 and Sephadex G-25. The single peak of anti-oxidized peptide was detected by mass spectrometry. The two components with molecular mass of 2 237 D and 3 372 D were rich A mixture of two oligopeptides containing aromatic and basic amino acids. The results suggest that Cordyceps militaris antioxidant peptide on mice due to radiation-induced changes in blood and oxidative damage has a repair effect.