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为了探讨一氧化氮合酶（ＮＯＳ）是否在肺内存在，取Ｗｉｓｔａｒ大鼠２０只，断颈处死取肺，冰冻切片，ＮＡＤＰＨＤ孵育液孵育，经洗，脱水、透明和封片，光镜观察ＮＯＳ阳性细胞。观察发现：在Ｗｉｓｔａｒ大鼠肺内的各级支气管上皮层内，含有ＮＯＳ阳性细胞。阳性细胞胞浆内含蓝色或深蓝色颗粒，核不着色。ＮＯＳ阳性细胞以细支气管和终末细支气管上皮层内最多且Ｃｌａｒａ细胞是ＮＯＳ阳性细胞。结果提示：肺内含有ＮＯＳ阳性细胞，这一发现为临床治疗支气管哮喘提供了理论依据
In order to investigate whether nitric oxide synthase (NOS) is present in the lungs, 20 Wistar rats were sacrificed and the lungs were harvested at the neck and frozen. The sections were incubated with NADPH-D, washed, dehydrated, NOS positive cells were observed by microscope. Observation found that: Wistar rat lungs at all levels within the bronchial epithelium, containing NOS positive cells. Positive cytoplasm contains blue or dark blue particles, the nucleus is not colored. Most NOS positive cells are in the bronchiolar and terminal bronchiolar epithelial layers and Clara cells are NOS positive cells. The results suggest that: the lung contains NOS positive cells, this finding provides a theoretical basis for the clinical treatment of bronchial asthma