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目的探讨前列腺炎Ⅰ号治疗慢性非细菌性前列腺炎的作用机制。方法将70只大鼠随机分为空白组、模型组、阳性组、前列腺炎Ⅰ号小剂量组、前列腺炎Ⅰ号中剂量组、前列腺炎Ⅰ号大剂量组。空白组皮下注射1 mL生理盐水,其余各组皮下多点注射前列腺蛋白提纯液与弗氏完全佐剂混合乳剂1 mL,30 d后重复造模1次,45 d后灌胃给药。连续给药4周后,剥取前列腺组织计算前列腺指数,提取血清检测白细胞介素-2(IL-2)、白细胞介素-6(IL-6)含量。结果与模型组大鼠血清IL-2、IL-6含量比较,其他各组均较低,差异有统计学意义(P<0.05);与阳性组大鼠血清IL-2、IL-6含量比较,前列腺炎Ⅰ号小剂量组较高,差异统计学意义(P<0.05);与前列腺炎Ⅰ号小剂量组大鼠血清IL-2、IL-6含量比较,前列腺炎Ⅰ号中、大剂量组更低,差异有统计学意义(P<0.05)。结论前列腺炎Ⅰ号中、大剂量能有效降低CNP大鼠模型血清IL-2、IL-6的表达水平,从而改善局部免疫功能,抑制炎症反应。
Objective To investigate the mechanism of prostatitis Ⅰ in the treatment of chronic nonbacterial prostatitis. Methods Seventy rats were randomly divided into blank group, model group, positive group, prostatitis Ⅰ low dose group, prostatitis Ⅰ medium dose group, prostatitis Ⅰ high dose group. 1 mL normal saline was injected subcutaneously in the blank group, and 1 mL subcutaneous injection of prostatic protein purification solution and Freund’s complete adjuvant mixed emulsion were injected into the other groups. After 30 d, the model was repeated 1 and 45 d later. Four weeks after continuous administration, prostatic tissue was stripped to calculate the serum levels of interleukin-2 (IL-2) and interleukin-6 (IL-6). Results Compared with the model group, the levels of IL-2 and IL-6 in serum of the model group were lower than those in the other groups, the difference was statistically significant (P <0.05); compared with the positive group, the levels of IL-2 and IL- , Prostatitis Ⅰ low dose group was higher, the difference was statistically significant (P <0.05); and prostatitis Ⅰ low dose group serum IL-2, IL-6 levels, prostatitis Ⅰ, the large dose Group was lower, the difference was statistically significant (P <0.05). Conclusions In prostatitis Ⅰ, high dose can effectively reduce the expression of IL-2 and IL-6 in CNP rat model, so as to improve the local immune function and inhibit the inflammatory reaction.