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用超声法制备大豆磷脂小单层脂质体,低渗溶血法制备绵羊红细胞膜。在一定条件下,将脂质体与红细胞膜温育一定时间后,离心去掉脂质体,用正己烷-异丙醇,提取膜总脂质,用高效液相色谱法分离测定温育前后膜脂组成。结果表明,温育后红细胞膜的磷脂酰胆碱与鞘磷脂物质的量之比(npc/nsM)明显增加,这是影响膜流动性的重要参数。上述结果与作者以前用DPH荧光偏振技术测定的同样条件下膜流动性变化完全一致。因此,很好地从分子水平上解释了与大豆磷脂脂质体温育后,红细胞膜流动性的增加。
Soybean lecithin small monolayer liposomes were prepared by ultrasonic method and sheep erythrocyte membrane was prepared by hypotonic hemolysis method. Under certain conditions, the liposomes were incubated with the erythrocyte membrane for a certain period of time, then the liposomes were removed by centrifugation and the total lipid was extracted with n-hexane-isopropanol. The membrane before and after the incubation was separated by high performance liquid chromatography Fat composition. The results showed that the ratio of phosphatidylcholine to sphingomyelin (npc / nsM) in the erythrocyte membrane increased significantly after incubation, which is an important parameter affecting membrane fluidity. The above results are in good agreement with the changes in membrane fluidity under the same conditions previously determined by the author using the DPH fluorescence polarization technique. Thus, it is well explained at the molecular level that the fluidity of erythrocyte membranes increases upon incubation with soya phospholipid liposomes.