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将保存于 M-K 溶液不同时间的人眼角膜,用扫描及透射电子显微镜作超微结构研究,结合锥蓝活体着色法和角膜穿透移植术后临床效果进行讨论。结果是,应用全眼球前房充气于 M-K 液保存的7只眼球中,保存时间最长者达138小时,角膜内皮表面和内皮细胞细胞器的超微结构均保持完整性,其中6个标本作穿透角膜移植术后,植片均为透明,提示全眼球前房充气保存法达到了中期保存材料的要求和有用时限,其优点是可以避免停滞的房水对内皮细胞的有害作用,保存前又不致于严重损害内皮细胞。保存于 M-K 液的11只游离角膜,其保存时间最长者达118小时,检查结果表明,超微结构在一周内可保持完整,与全眼球保存者无显著差别,其中9只游离保存角膜用于穿透角膜移植术,术后除2例是由于手术合并症等原因致植片混浊外,其余植片均透明。本文结果表明,全眼球前房充气和游离角膜均可以在 M-K 液中保存118～138小时,超微结构可保持相当的完整性,可用于穿透角膜移植手术。
The human corneas preserved in M-K solution at different times were examined by scanning electron microscopy (TEM) and transmission electron microscopy (TEM), and the clinical effects of cone blue staining and penetrating keratoplasty were discussed. The result is that the use of whole-eye anterior chamber inflated in the MK liquid preserved seven eyes, the longest preservation of up to 138 hours, the corneal endothelial surface and endothelial cell organelle ultrastructure remain intact, of which 6 specimens for wear Permeable membrane transplantation, the graft is transparent, suggesting that the whole eye anterior chamber inflation method to achieve the medium-term preservation of materials requirements and useful time limit, which has the advantage that stagnant aqueous humor can prevent the harmful effects of endothelial cells before preservation Do not cause serious damage to endothelial cells. Eleven preserved corneas preserved in MK fluid preserved for a maximum of 118 hours. The results showed that ultrastructural integrity remained intact in one week, with no significant difference from those preserved by the whole eye. Of the 9 preserved corneas In penetrating keratoplasty, except for 2 cases after surgery due to complications such as opacification caused by opacification, the rest were transparent. Our results show that the whole eye anterior chamber inflatable and free corneal can be stored in M-K liquid 118 to 138 hours, the ultrastructure can maintain a fairly complete, can be used for penetrating keratoplasty.