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目的 研究人脑星形胶质细胞瘤SHG 4 4细胞表达 (β 1,4 半乳糖基转移酶Ⅴ (beta 1,4 galactosyltransferaseⅤ ,β 1,4 GalTⅤ )后对化疗药物敏感性的影响 ,分析鬼臼乙叉甙 (VP16 )处理表达 β 1,4 半乳糖基转移酶Ⅴ的SHG 4 4细胞后其细胞粘附作用相关因子水平的变化。方法 用 12 0 μmol/L的VP16处理转染了正义、反义 β 1,4 GalTⅤ和空载体的SHG 4 4细胞 2 4h后 ,采用westernblot检测整合蛋白 β1表达变化及局部粘着斑激酶 (focaladhesionkinase,FAK)的蛋白水平和FAK的酪氨酸磷酸化水平。结果 在未经VP16处理的 3株SHG 4 4细胞中 ,FAK的蛋白水平没有明显改变 ,而整合蛋白 β1的蛋白表达在转正义 β 1,4 GalTⅤ的细胞中最高 ;经VP16处理后 ,SHG 4 4细胞中整合蛋白β1的表达水平增高 ,FAK及其磷酸化水平下降。但整合蛋白β1,FAK及其磷酸化水平随细胞中 β 1,4 GalTⅤ表达水平不同而变化。 结论 VP16处理对表达不同水平的β 1,4 GalT Ⅴ的SHG 4 4胶质瘤细胞的粘附影响有所不同 ,提示胶质瘤表达 β 1,4 GalTⅤ不仅与肿瘤的恶性行为有关 ,而且与肿瘤的化疗敏感性有关。
Objective To study the effect of β 1,4-galactosyltransferase Ⅴ (β 1,4-GalTⅤ) on the chemosensitivity of human astrocytoma SHG 4 4 cells. Changes of cell adhesion factors in SHG 4 4 cells expressing β 1,4-galactosyltransferase Ⅴ after treatment with orthotropism glycosides (VP16) .Methods The cells were transfected with VP16 (120 μmol / L) , Antisense β 1,4 GalT Ⅴ, and SHG 4 4 cells in empty vector for 24 hours. Western blot was used to detect the expression of integrin β1 and the protein level of FAK and FAK tyrosine phosphorylation Results The protein level of FAK did not change significantly in 3 SHG 4 4 cells without VP16, while the protein expression of integrin β1 was the highest in the cells transfected with sense β 1,3 and GalTⅤ. After VP16 treatment, SHG However, the levels of integrin β1, FAK and their phosphorylation varied with the level ofβ1, GalTⅤ in cells.4 Conclusion The expression of integrin β1 and the phosphorylation level of FAK decreased, The effect on the adhesion of SHG 4 4 glioma cells expressing different levels of β 1,4 GalT Ⅴ was different, suggesting that the expression of β 1,4 GalTⅤ in glioma is not only related to the malignant behavior of tumor, but also to the chemotherapy of tumor Sensitivity related.