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B7-H3在结直肠癌中的表达:其核定位可充分预测结肠癌的不良预后预测结直肠癌术后复发可能性的方法除了组织病理学分级外,还需要分子标志物予以补充。B7-H3作为一种免疫调节蛋白,数种癌症病程中均可见其过表连,且常与疾病晚期和不良预后相关。本研究旨在考察B7-H3是否可以作为一种潜在的结直肠癌预后标志物。对取自277例患者的结直肠癌石蜡包埋组织样本进行抗B7-H3抗体的免疫抗体标记。观察结果显示样本中分别有62%和46%的肿瘤细胞质和细胞膜中可见B7-H3表达。不出意外,B7-H3也可见于30%的肿瘤细胞核。亚细胞结构的Western免疫印迹分析结果也证实了上述核定位结果。关键的是,结肠癌中,B7-H3在细胞核内的表达与无转移生存率、疾病特异性生存率以及总体生存率三者的下降呈独立且显著的相关关系。大部分样本在肿瘤相关脉管系统和成纤维细胞
Expression of B7-H3 in colorectal cancer: its nuclear localization can fully predict the unfavorable prognosis of colon cancer. Methods of predicting the possibility of postoperative recurrence of colorectal cancer require molecular markers in addition to histopathological grade. As an immunomodulatory protein, B7-H3 is overexpressed in several cancers and is often associated with advanced disease and poor prognosis. The aim of this study was to investigate whether B7-H3 may serve as a potential prognostic marker of colorectal cancer. Colorectal cancer paraffin-embedded tissue samples taken from 277 patients were immunized with anti-B7-H3 antibody. Observations showed that 62% and 46% of the samples had cytoplasmic and B7-H3 expression in the cell membrane, respectively. Not surprisingly, B7-H3 is also found in 30% of the tumor nuclei. Western immunoblot analysis of the subcellular structure also confirmed the above nuclear localization results. Crucially, in colon cancer, the expression of B7-H3 in the nucleus was independently and significantly correlated with the decline of non-metastatic survival, disease-specific survival and overall survival. Most of the samples are in tumor-associated vasculature and fibroblasts