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本文以温室三年生喜树腋芽为外植体,研究了不同的基本培养基和基本培养基中添加不同浓度生长调节物质(BA 或TDZ)、蔗糖、琼脂以及培养基的pH 值对喜树腋芽分化的影响。结果表明:WPM 和B5 培养基适合喜树腋芽的诱导,MS 培养基不利于喜树腋芽的诱导。适合喜树腋芽增殖和分化的最佳生长调节物质为BA 1.0mg/L 或TDZ 0.1mg/L;最佳的蔗糖浓度为30g/L;最佳的琼脂浓度为6g/L;pH5.8-6.6 的范围均适合喜树腋芽的诱导,但最佳的pH 值为5.8。图1 表5 参14。
In this paper, the axillary buds of Camptotheca acuminatum in greenhouse were used as explants to study the effects of different concentrations of growth regulator (BA or TDZ), sucrose, agar and medium on the basic and medium The impact of differentiation. The results showed that WPM and B5 medium were suitable for induction of axillary buds of Camptotheca acuminata. MS medium was not conducive to the induction of axillary buds. The optimum growth regulators suitable for axillary bud proliferation and differentiation were BA 1.0 mg / L or TDZ 0.1 mg / L, the best sucrose concentration was 30 g / L, the best agar concentration was 6 g / L, pH 5.8- The range of 6.6 is suitable for induction of axillary buds of Camptotheca acuminata, but the optimum pH value is 5.8. Figure 1 Table 5 参 14.