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用系列单抗与标记的花生凝集素（ＰＮＡ）对３８例急性淋巴细胞白血病（ＡＬＬ）细胞行免疫分型与确定分化阶段，检测ＡＬＬ细胞ＰＮＡ受体表达情况，与正常成熟淋巴细胞（ＮＭＬ）对照。发现Ｔ－ＡＬＬ，Ｎｏｎ－Ｔ－ＡＬＬ细胞大多表达ＰＮＡ受体，而ＮＭＬ细胞极少表达ＰＮＡ受体，两者有显著差别（Ｐ＜０．０１）。经神经氨酸酶（ＮＡ）预处理后，Ｎｏｎ－Ｔ－ＡＬＬ与ＮＭＬ细胞ＰＮＡ受体表达明显增加（Ｐ＜０．０５），以ＮＭＬ为甚，而Ｔ－ＡＬＬ细胞ＮＡ处理前后ＰＮＡ受体表达未见明显区别；ＡＬＬ细胞ＰＮＡ受体表达与细胞分化程度存在显著的等级相关（ｒ８＝－０．３５７，Ｐ＜０．０５）。结果表明ＡＬＬ细胞上大多存在Ｄ－半乳糖－β（１－３）－Ｎ－乙酰氨基半乳糖基（Ｄ－Ｇａｌ－β（１－３）－Ｎ－ＧａｌＮＡＣ）；ＡＬＬ细胞分化程度越高。细胞上Ｄ－Ｇａｌ－β（１－３）－Ｎ－ＧａｌＮＡＣ连接唾液酸末端越多。
38 cases of acute lymphoblastic leukemia (ALL) were immunophenotyping and differentiation stage with series of monoclonal antibodies and labeled peanut agglutinin (PNA). The expression of PNA receptor in ALL cells was detected and compared with normal mature lymphocytes (NML) Control. Found that T-ALL, Non-T-ALL cells mostly express PNA receptor, while NML cells rarely express PNA receptor, both significant differences (P <0.01). After neuraminidase (NA) pretreatment, the expression of PNA receptor in Non-T-ALL and NML cells was significantly increased (P <0.05), especially in NML, while PNA receptor in T-ALL cells before and after NA treatment There was no significant difference between the expression of PNA receptor and cell differentiation in ALL cells (r8 = -0.357, P <0.05). The results showed that most of the cells on the cell D-galactose-β (1-3) -N-acetylgalactosaminyl (D-Gal-β (1-3) -N-GalNAC); ALL cells higher degree of differentiation. The more D-Gal-beta (1-3) -N-GalNAC on the cell is attached to the sialic acid end.